RFP Labelled Mouse (129) Embryonic Stem Cell - NIH/3T3

Name: RFP Labelled Mouse (129) Embryonic Stem Cell - NIH/3T3
SKU: CSC-RR0422
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR0422

Host Cell : NIH3T3 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RR0422
Description	This cell line is engineered to stably overexpress the RFP in Mouse Embryonic Stem Cell cells.
Introduction	This product is derived from the inner cell mass of specific pathogen-free (SPF) strain 129 mouse blastocysts, which is transfected with a lentiviral vector encoding RFP. Cells are positive for alkaline phosphatase staining and are able to differentiate into cell types of all three germ layers. Using the recommended media and protocol, cells can be passaged for several times. It is a good cell model for stem cell studies.
Target Gene	RFP
Host Cell	NIH3T3
Host Cell Species	Mus musculus (Mouse)
Reporter Type	Fluorescent protein
Applications	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Target Gene

RFP

Background

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq, Jul 2008]

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