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Cat.No. : CSC-RR0418 Host Cell: Rat (F344) Mesenchymal Stem Cell
| Cat. No. | CSC-RR0418 |
| Description | This cell line is engineered to stably overexpress the RFP in Rat (F344) Mesenchymal Stem Cell cells. |
| Introduction | This product is derived from Fischer 344 (F344) rat bone marrow, which is transfected with a lentiviral vector encoding RFP. It maintains the multipotent differentiation ability. Flow cytometry assay shows that these cells are positive for CD44, CD90 (>70%), and negative for CD34, CD11b and CD45 (<5%). it="" is="" a="" good="" cell="" model="" for="" stem="" cell="" studies.="" using="" the="" recommended="" media="" and="" protocol,="" cells="" can="" be="" cultured="" more="" than="" 5=""> |
| Gene | RFP |
| Host Cell | Rat (F344) Mesenchymal Stem Cell |
| Host Cell Species | Rattus norvegicus (Rat) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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