Cat.No. : CSC-RO0102 Host Cell: T47D
Size: >1x10^6 frozen cells/vial, 1 mL Stability: Stable in culture over a minimum of 10 passages
| Cat. No. | CSC-RO0102 |
| Description | T47D-ERBB2 cell line is a stably transfected cell line which expresses human erb-b2 receptor tyrosine kinase 2 (ERBB2). |
| Target Gene | ERBB2 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | T47D |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Size | >1x10^6 frozen cells/vial, 1 mL |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Emphasizing the important function of Nectin-4 in cancer, it is well known that this protein interacts with ErbB2, a major factor in the development of breast cancer, and is elevated in many other malignancies. To look at the cooperative impact of Nectin-4 and ErbB2, the researchers used human breast cancer T47D cells endogenously expressing both of these proteins. They specifically examined how the combination of Nectin-4 and p95-ErbB2 enhances SOX2 gene expression and promotes cell proliferation in suspension culture. Their findings indicated that this cooperation activates the PI3K-AKT signaling pathway, a crucial mechanism for cell growth. By establishing various stable T47D cell lines expressing different combinations of ErbB2 and Nectin-4, they were able to quantify the proliferation rates under anchorage-independent conditions.
Figure 1. The researchers cultured T47D cells in RPMI-1640 medium and employed electroporation to introduce plasmids for stable expression of Nectin-4 and ErbB2, aiming to investigate their roles in enhancing cell proliferation and SOX2 expression. (Kedashiro S, et al., 2021)
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The Human ERBB2 Stable Cell Line-T47D is crafted with high precision, ensuring specific and targeted modifications to the ERBB2 gene. This accuracy is crucial for studying the function of ERBB2 in cancer development and testing potential therapeutic interventions.
The genetic modifications in the Human ERBB2 Stable Cell Line-T47D are designed to be stable over numerous cell divisions, providing a consistent and reliable model for long-term experiments. This durability is essential for researchers conducting extended studies on ERBB2-related biology.
The Human ERBB2 Stable Cell Line-T47D is well-suited for large-scale research projects, as it can be easily expanded and maintained in culture. This scalability allows researchers to perform high-throughput screens, molecular analyses.
The Human ERBB2 Stable Cell Line-T47D offers a cost-effective solution for researchers, as it eliminates the need for frequent gene editing and cell line generation. The stable nature of this cell line reduces costs associated with reagents, labor, and time, making it an efficient choice for ERBB2-related research.
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