Human ERBB2 Stable Cell Line-Ba/F3

Name: Human ERBB2 Stable Cell Line-Ba/F3
SKU: CSC-RO0103
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0103

Host Cell : Ba/F3 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0103
Description	Ba/F3-ERBB2 cell line is a stably transfected cell line which expresses human erb-b2 receptor tyrosine kinase 2 (ERBB2).
Target Gene	ERBB2
Gene Species	Homo sapiens (Human)
Host Cell	Ba/F3
Host Cell Species	Mus musculus (Mouse)
Applications	Drug screening and biological assays
Biosafety Level	2
Size	>1x10^6 frozen cells/vial, 1 mL
Stability	Stable in culture over a minimum of 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Growth Conditions	37 °C, 5% CO₂
Shipping	Dry ice

Thawing & Subculturing Instructions	1. Thaw cells by gently swirling in a 37°C water bath. To limit contamination, do not submerge the O-ring and cap. 2. When cells are ~70% thawed (~1 min), transfer the vial into a biosafety cabinet, and wipe the surface with 70% ethanol. Allow tube to dry completely. 3. Transfer the cells gently into a 15 mL conical tube containing 10 mL of pre-warmed culture medium (without antibiotic selection marker). Centrifuge cells at ~125 x g for 5~7 min. 4. Remove supernatant without disturbing the pellet, and resuspend cells in 1 mL culture medium (without antibiotic selection marker). Transfer cells to a 6-well plate containing ~2 mL pre-warmed growth medium (without antibiotic selection marker) or a T25 flask containing 5 mL pre-warmed culture medium (without antibiotic selection marker). 5. Incubate the culture at 37°C with 5% CO2. 6. Subculture: split saturated culture 1:4 ~ 1:6 every 3 days; seed out at about 1~3 x 10^5 cells/mL.
Freeze Medium	Frozen with 70% medium, 20% FBS, 10% DMSO
Growth Properties	Suspension, round
Freezing Instructions	Cells are recommended to generate additional frozen stocks at early passages. Frozen stocks should be preserved in a designated cryopreservation medium or in 70% RPMI 1640 + 20% FBS + 10% DMSO (without antibiotic selection marker). 1. Prepare the freezing medium (70% RPMI 1640 + 20% FBS + 10% DMSO, without antibiotic selection marker) fresh immediately before use. 2. Keep the freezing medium on ice and label cryovials. 3. Transfer cells to a sterile, conical centrifuge tube, and count the cells. 4. Centrifuge the cells at 250 x g for 5 minutes at room temperature and carefully aspirate off the medium. 5. Resuspend the cells at a density of at least 3 x10^6 cells/ml in chilled freezing medium. 6. Aliquot 1 ml of the cell suspension into each cryovial. 7. Freeze cells in the CoolCell freezing container overnight in a -80°C freezer. 8. Transfer vials to liquid nitrogen for long-term storage.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ERBB2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) [ Homo sapiens ]
Gene Symbol	ERBB2
Synonyms	NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Gene Description	v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Gene ID	2064
Uni Prot ID	P04626
m RNA Refseq	NM_001005862.1
Protein Refseq	NP_001005862.1
Chromosome Location	17q12
Function	ATP binding; ErbB-3 class receptor binding; RNA polymerase I core binding; epidermal growth factor-activated receptor activity; contributes_to growth factor binding; identical protein binding; protein C-terminus binding; protein binding; protein dimerization activity; protein heterodimerization activity; protein heterodimerization activity; protein heterodimerization activity; protein phosphatase binding; protein tyrosine kinase activity; protein tyrosine kinase activity; protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; transmembrane signaling receptor activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Axon guidance, organism-specific biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem;
MIM	164870

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Background

Applications

Q & A

Customer Reviews

The Human ERBB2 gene, also known as HER2 or NEU, encodes a receptor tyrosine kinase that is a member of the epidermal growth factor receptor (EGFR) family. ERBB2 is overexpressed or amplified in various cancers, including breast and ovarian cancer, and its overexpression is associated with poor prognosis and aggressive disease.

The Ba/F3 cell line, derived from a human acute lymphoblastic leukemia, is a widely used model for studying the function of tyrosine kinase receptors and their signaling pathways. When engineered to stably express the human ERBB2 gene, the Ba/F3 cell line becomes a valuable tool for investigating the role of ERBB2 in cancer cell growth, survival, and response to targeted therapies, such as trastuzumab (Herceptin), which is a monoclonal antibody that targets HER2.

The Human ERBB2 Stable Cell Line-Ba/F3 is a cell line that expresses the ERBB2 (HER2) gene, which encodes a receptor tyrosine kinase involved in cell growth and differentiation. This cell line is particularly useful for studying the effects of ERBB2 overexpression in cancer and for testing targeted therapies.

（1）Cancer Therapy Research:

ERBB2 amplification and overexpression are associated with aggressive forms of cancer, such as breast and ovarian cancer. This cell line can be used to test the efficacy of ERBB2-targeted therapies, including tyrosine kinase inhibitors and monoclonal antibodies, which can help in the development of more effective cancer treatments.

（2） Signaling Pathway Analysis:

The Ba/F3 ERBB2 Stable Cell Line can be employed to study the downstream signaling pathways activated by ERBB2, such as the PI3K/AKT and MAPK/ERK pathways. Understanding these pathways is crucial for identifying potential therapeutic targets and developing strategies to disrupt cancer cell signaling.

（3）Drug Resistance Mechanisms:

ERBB2-overexpressing cells can be used to investigate the mechanisms of resistance to ERBB2-targeted therapies. By studying the adaptive changes in these cells, researchers can identify new targets for overcoming resistance and improving treatment outcomes.

Customer Q&As

What specific signaling pathways can be investigated using the Human ERBB2 Stable Cell Line-Ba/F3, and how does the overexpression of ERBB2 in this cell line impact these pathways?

A: The Human ERBB2 Stable Cell Line-Ba/F3 allows for the investigation of multiple signaling pathways downstream of the ERBB2 receptor, including the PI3K/AKT, MAPK/ERK, and PLCγ pathways. The overexpression of ERBB2 in these cells leads to the activation of these pathways, which play crucial roles in cell growth, survival, and differentiation. By utilizing this cell line, researchers can study the effects of various inhibitors or activators on these pathways, providing insights into the mechanisms of ERBB2-driven cell signaling and potential targets for cancer therapy.

How can the Human ERBB2 Stable Cell Line-Ba/F3 be used to assess the efficacy of ERBB2-targeted therapies, such as monoclonal antibodies and tyrosine kinase inhibitors?

A: The Human ERBB2 Stable Cell Line-Ba/F3 is an excellent model for evaluating the efficacy of ERBB2-targeted therapies due to its reliance on ERBB2 signaling for growth and survival. Researchers can treat these cells with different concentrations of ERBB2-targeted agents and assess their effects on cell viability, proliferation, and signaling. This can provide valuable information on the potency and specificity of these therapeutics, as well as help optimize dosing and treatment regimens for clinical applications.

What are the key experimental considerations when using the Human ERBB2 Stable Cell Line-Ba/F3 to study the effects of genetic mutations on ERBB2 function and signaling?

A: When using the Human ERBB2 Stable Cell Line-Ba/F3 to study the impact of genetic mutations on ERBB2 function, it is important to introduce these mutations using precise genetic engineering techniques, such as CRISPR/Cas9 or site-directed mutagenesis. Researchers must also consider the potential off-target effects of these techniques and validate the impact of the mutations on ERBB2 activity and downstream signaling pathways through various assays, including Western blotting, immunoprecipitation, and reporter gene assays.

How can the Human ERBB2 Stable Cell Line-Ba/F3 facilitate the identification of novel ERBB2 interactors and their roles in cellular processes?

A: The Human ERBB2 Stable Cell Line-Ba/F3 can be used to perform co-immunoprecipitation experiments to identify novel interactors of the ERBB2 receptor. By expressing tagged versions of ERBB2 or its potential interactors, researchers can isolate protein complexes and identify novel binding partners. Further functional studies, such as knockdown or overexpression experiments, can then be conducted to determine the roles of these interactors in cellular processes, such as signal transduction, cell cycle regulation, and apoptosis.

What is the potential application of the Human ERBB2 Stable Cell Line-Ba/F3 in the development of resistance mechanisms to ERBB2-targeted therapies, and how can this information be used to improve treatment strategies?

A: The Human ERBB2 Stable Cell Line-Ba/F3 can be employed to study the development of resistance to ERBB2-targeted therapies by long-term exposure to these agents, leading to the selection of resistant cell sublines. These resistant cells can then be analyzed to identify the molecular mechanisms underlying the resistance, such as alterations in ERBB2 expression levels, activation of alternative signaling pathways, or changes in drug metabolism. This information can be used to develop strategies to overcome or prevent resistance, ultimately improving the efficacy of ERBB2-targeted therapies.

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Customer Reviews

Enhanced ERBB2 Protein Study

The Human ERBB2 Stable Cell Line - Ba/F3 enables enhanced study of the ERBB2 protein, a key receptor tyrosine kinase involved in cell growth and differentiation. This cell line provides a consistent and controlled environment to explore ERBB2's role in oncogenic processes, making it invaluable for cancer research.

United Kingdom

2020-03-05

Supports Signal Pathway Elucidation

By stably expressing ERBB2, the Human ERBB2 Stable Cell Line - Ba/F3 assists in elucidating signaling pathways that are activated by ERBB2 engagement. This is critical for understanding the molecular mechanisms of diseases where ERBB2 is implicated, such as breast and ovarian cancers.

United States

2021-05-15

Improved Reproducibility in Experiments

The consistent expression of ERBB2 in the Human ERBB2 Stable Cell Line - Ba/F3 enhances the reproducibility of experimental results. This reproducibility is crucial for studies that require precise, reliable data, particularly in preclinical cancer research.

French

2022-07-06

Versatile Application in Functional Assays

The Human ERBB2 Stable Cell Line - Ba/F3 is versatile, suitable for a range of functional assays including proliferation, apoptosis, and cell signaling studies. This versatility makes it an essential tool for comprehensive analysis of ERBB2 functions and its contributions to pathological conditions.

United States

2020-03-16

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Human ERBB2 Stable Cell Line-Ba/F3

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Applications

Q & A

Customer Reviews

What specific signaling pathways can be investigated using the Human ERBB2 Stable Cell Line-Ba/F3, and how does the overexpression of ERBB2 in this cell line impact these pathways?

How can the Human ERBB2 Stable Cell Line-Ba/F3 be used to assess the efficacy of ERBB2-targeted therapies, such as monoclonal antibodies and tyrosine kinase inhibitors?

What are the key experimental considerations when using the Human ERBB2 Stable Cell Line-Ba/F3 to study the effects of genetic mutations on ERBB2 function and signaling?

How can the Human ERBB2 Stable Cell Line-Ba/F3 facilitate the identification of novel ERBB2 interactors and their roles in cellular processes?

What is the potential application of the Human ERBB2 Stable Cell Line-Ba/F3 in the development of resistance mechanisms to ERBB2-targeted therapies, and how can this information be used to improve treatment strategies?

Enhanced ERBB2 Protein Study

Supports Signal Pathway Elucidation

Improved Reproducibility in Experiments

Versatile Application in Functional Assays

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