scFv(HER2)-CD28-CD3zeta CAR-T Lentivirus

scFv(HER2)-CD28-CD3ζ CAR-T Lentivirus is a gene-delivery reagent designed to produce T cells that recognize human epidermal growth factor receptor 2 (HER2/ERBB2). The encoded second-generation chimeric antigen receptor combines an extracellular anti-HER2 single-chain variable fragment (scFv) with membrane-anchoring elements, the intracellular costimulatory domain of CD28, and the CD3ζ activation domain. The scFv directs antibody-like binding to HER2 on the cell surface without dependence on major histocompatibility complex presentation. Following target engagement, CD3ζ delivers the primary activation signal, while CD28 provides costimulation that supports T-cell activation, proliferation, cytokine production, and effector function. Lentiviral delivery enables efficient gene transfer and stable CAR expression in transduced T cells, providing a practical and reproducible system for generating HER2-responsive cells for laboratory studies.

This product is intended for research on HER2-targeted cellular immunotherapy and the functional characterization of CD28-based CAR-T cells. It can be used to prepare experimental CAR-T cells for measuring receptor expression, antigen-specific activation, cytotoxicity, cytokine secretion, proliferation, and changes in differentiation or exhaustion markers. Comparisons among HER2-high, HER2-low, and HER2-negative target cells can help define antigen-density requirements, selectivity, and potential on-target effects in tissues with different HER2 levels. The resulting cells are suitable for studies involving HER2-expressing breast, gastric, ovarian, lung, and other solid-tumor models in appropriately designed in vitro or preclinical systems. This construct may also serve as a reference for comparing CD28 signaling with first-generation CARs or receptors containing alternative costimulatory domains such as 4-1BB. Additional applications include investigating repeated antigen exposure, tumor-cell resistance, immune escape, and suppression within the tumor microenvironment. The lentiviral format helps streamline exploratory screening, mechanistic research, and early evaluation of combination approaches involving targeted therapies, checkpoint inhibitors, cytokines, or other immunomodulatory agents.