scFv(HER2)-CD28-CD3zeta CAR-T Lentivirus

Name: scFv(HER2)-CD28-CD3zeta CAR-T Lentivirus
SKU: LVG00051Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : LVG00051Z

Storage : -80℃ Shipping : Frozen on dry ice

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Gene Information

Cat. No.	LVG00051Z
Description	Lentivirus particles containing second generation of anti-HER2 CAR (chimeric antigen receptor) scFv-CD28-CD3zeta.
Gene	ERBB2
Titer	Varies lot by lot, for example, ≥110^7 TU/mL, ≥110^8 TU/mL, ≥1*10^9 TU/mL etc.
Size	Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma	Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity	Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility	The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation	All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.

Gene Name	ERBB2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) [ Homo sapiens ]
Gene Symbol	ERBB2
Synonyms	NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Gene Description	v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Gene ID	2064
Uni Prot ID	P04626
m RNA Refseq	NM_001005862.1
Protein Refseq	NP_001005862.1
Chromosome Location	17q12
Function	ATP binding; ErbB-3 class receptor binding; RNA polymerase I core binding; epidermal growth factor-activated receptor activity; contributes_to growth factor binding; identical protein binding; protein C-terminus binding; protein binding; protein dimerization activity; protein heterodimerization activity; protein heterodimerization activity; protein heterodimerization activity; protein phosphatase binding; protein tyrosine kinase activity; protein tyrosine kinase activity; protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; transmembrane signaling receptor activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Axon guidance, organism-specific biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem;
MIM	164870

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High Titer

Impressed by the high functional titer! We achieved efficient CAR expression in primary human T-cells using a relatively low MOI. This not only saved precious virus but also minimized potential cytotoxicity from the transduction process itself.

French

2022-06-20

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scFv(HER2)-CD28-CD3zeta CAR-T Lentivirus

Virus Particles Information

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High Titer

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