scFv(HER2)-CD3zeta CAR-T Lentivirus

scFv(HER2)-CD3ζ CAR-T Lentivirus is a gene-delivery reagent designed to generate T cells that recognize human epidermal growth factor receptor 2 (HER2/ERBB2). The encoded chimeric antigen receptor combines an extracellular anti-HER2 single-chain variable fragment (scFv) with membrane-anchoring elements and the intracellular signaling domain of CD3ζ. The scFv provides antibody-like recognition of cell-surface HER2 without requiring peptide presentation by the major histocompatibility complex, while CD3ζ initiates T-cell activation following receptor engagement. Because this construct contains CD3ζ without an additional costimulatory domain, it represents a first-generation CAR design. Lentiviral delivery enables efficient gene transfer into dividing and non-dividing cells and supports stable CAR expression in transduced T cells, providing a consistent starting point for downstream functional studies.

This product is intended for research into HER2-directed cellular immunotherapy and the biology of antigen-specific T-cell responses. It can be used to prepare experimental CAR-T cells for assessing receptor expression, HER2-dependent activation, target-cell killing, cytokine release, proliferation, and changes in T-cell phenotype. Researchers may compare responses across HER2-high, HER2-low, and HER2-negative cell models to examine antigen density, specificity, and potential off-target activity. The resulting cells are also suitable for evaluating HER2-positive tumor models, including models derived from breast, ovarian, gastric, and other solid tumors in which HER2 expression is relevant. As a first-generation construct, scFv(HER2)-CD3ζ CAR-T Lentivirus can serve as a useful reference when studying the contribution of costimulatory domains or comparing alternative CAR architectures. It may also support preclinical investigations of combination strategies, tumor-cell resistance, immune escape, and the influence of the tumor microenvironment on CAR-T-cell function.