Human ERBB2 Stable Cell Line - A549

Name: Human ERBB2 Stable Cell Line - A549
SKU: CSC-RO01351

Cat.No. : CSC-RO01351 Host Cell: Human non-small cell lung carcinoma / cancer cell line (A549)

Size: >1x10^6 frozen cells/vial, 1 mL Stability: Stable in culture over a minimum of 10 passages

Inquire for Price

Cell Line Information

Safety and Packaging

Gene Informationn

Cat. No.	CSC-RO01351
Description	This cell line is engineered to stably express Homo sapiens (human) erb-b2 receptor tyrosine kinase 2 (ERBB2) in Human non-small cell lung carcinoma / cancer cell line (A549).
Target Gene	ERBB2
Gene Species	Homo sapiens (human)
Host Cell	Human non-small cell lung carcinoma / cancer cell line (A549)
Host Cell Species	Homo sapiens (human) cell line
Stability	Stable in culture over a minimum of 10 passages
Product Type	Human gene overexpression stable cell line
Application	Drug screening and biological assays
Applications	1) investigation of gene function 2) screening and validation of antibodies
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Growth Properties	Adherent
Size Form	One vial of frozen cells, typically >1x10^6cells/vial
Shipping	Dry ice
Storage	Liquid nitrogen
Size	>1x10^6 frozen cells/vial, 1 mL

Gene Name	ERBB2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) [ Homo sapiens ]
Gene Symbol	ERBB2
Synonyms	NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Gene Description	v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Gene ID	2064
UniProt ID	P04626
mRNA Refseq	NM_001005862.1
Protein Refseq	NP_001005862.1
Chromosome Location	17q12
Function	ATP binding; ErbB-3 class receptor binding; RNA polymerase I core binding; epidermal growth factor-activated receptor activity; contributes_to growth factor binding; identical protein binding; protein C-terminus binding; protein binding; protein dimerization activity; protein heterodimerization activity; protein heterodimerization activity; protein heterodimerization activity; protein phosphatase binding; protein tyrosine kinase activity; protein tyrosine kinase activity; protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; transmembrane signaling receptor activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Axon guidance, organism-specific biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem;
MIM	164870

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Quick Inquiry

Background

Publications

Q & A

Customer Reviews

The ERBB2 gene (HER2) encodes a transmembrane receptor tyrosine kinase belonging to the epidermal growth factor receptor family. The ERBB2 signaling pathway plays a crucial role in the growth and survival of various epithelial tumors, particularly those with gene amplification. In non-small cell lung cancer (NSCLC), ERBB2 mutations or amplifications are associated with poor prognosis and resistance to conventional EGFR inhibitors. Constitutive activation of HER2 leads to persistent activation of the Ras/Raf/MAPK and PI3K/Akt pathways, which are major drivers of oncogenic transformation.

A stable human ERBB2 cell line constructed in the A549 context provides a powerful platform for studying the HER2 signaling pathway in lung adenocarcinoma. A549 cells are well-characterized human alveolar epithelial cells widely used in drug screening and oncology research. By stably overexpressing human ERBB2, this cell line allows researchers to analyze how enhanced HER2 activity modulates the metabolic and invasive behavior of lung cancer cells. This product is particularly suitable for high-throughput screening of HER2-targeting tyrosine kinase inhibitors (TKIs) and for studying acquired resistance mechanisms in non-small cell lung cancer (NSCLC).

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

First Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *