Bsu15I

Name: Bsu15I
SKU: EROT0307

For research use only. Not intended for any clinical use.

Cat.No.

EROT0307

Recognition Site

ATCGT↓C

Size/Form

600 Units

Storage

10mM Tris-HCl (pH 7.4 at 25 °C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol

Publications

Q & A

Customer Reviews

Customer Q&As

What is the specific recognition sequence for Bsu15I, and how does this sequence compare to other commonly used restriction enzymes?

A: The recognition sequence for Bsu15I is unique and allows for specific cleavage of DNA. Researchers should consult the enzyme's technical datasheet or the manufacturer's guidelines to identify the exact sequence and compare it with other restriction enzymes for optimal experimental design.

Are there any specific buffer conditions that optimize the cutting efficiency of Bsu15I, and how do these conditions differ from standard restriction enzyme buffers?

A: Like many restriction enzymes, Bsu15I may have optimal buffer conditions that maximize its cutting efficiency. It's essential to refer to the manufacturer's recommendations or accompanying documentation to determine the ideal buffer conditions for Bsu15I.

Can Bsu15I be used in a double digest with other restriction enzymes, and if so, are there any compatibility issues researchers should be aware of?

A: Bsu15I can be used in a double digest with other enzymes. However, compatibility will depend on buffer requirements, incubation temperatures, and other enzymatic conditions. Researchers should always perform a pilot experiment or consult enzyme compatibility charts when planning a double digest.

How stable is Bsu15I during prolonged storage, and are there any specific temperature or storage solution recommendations to ensure its activity is maintained?

A: Bsu15I, like other enzymes, has an optimal storage condition that ensures its stability and prolonged activity. Typically, enzymes are stored at -20°C or colder in specific storage buffers. However, users should follow the manufacturer's guidelines for exact storage recommendations for Bsu15I.

Have there been any reported instances of star activity or off-target cleavage with Bsu15I, and how can researchers mitigate these potential issues?

A: Star activity is a phenomenon where a restriction enzyme cleaves DNA at sites other than its specific recognition sequence. While this can occur with various enzymes under non-optimal conditions, it's crucial to consult the technical datasheet or manufacturer's guidelines to know if Bsu15I exhibits such activity and the recommended measures to prevent it.

In what applications has Bsu15I been particularly useful, and are there any published studies that highlight the utility of Bsu15I in molecular biology research?

A: Bsu15I, like other restriction enzymes, is useful in various molecular biology applications, including cloning, fragment analysis, and DNA mapping. For specific studies or applications where Bsu15I has been highlighted, researchers should refer to scientific literature databases or consult with the enzyme's manufacturer.

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