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Bsh1236I

For research use only. Not intended for any clinical use.
Cat.No.
EROT0268
Reaction Buffer
10mM Tris-HCl (pH 8.5 at 37 °C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
Reaction Conditions
Incubate at 37 °C
Recognition Site
CG↓CG
Size/Form
500 Units;1000 units
Storage
10mM potassium phosphate (pH 7.5 at 25 °C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Publications

Q & A

Customer Reviews

Customer Q&As
What is the composition of the reaction buffer of the enzyme?

A: The reaction buffer of the enzyme is 10mM Tris-HCl, 10mM MgCl2, 100mM KCl, and 0.1mg/mL BSA.

How long does the endonuclease usually need to cut?

A: The enzyme is generally digested at 37°C for 2-4 hours or overnight.

Is the endonuclease sensitive to methylation?

A: The enzyme is not sensitive to DAM methylation or DCM methylation, but it is sensitive to CpG methylation.

Is this product authentic?

A: We have not encountered any issues when using the enzyme, and the success rate of digestion is high. The enzyme can cut the target sequence in most cases.

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Customer Reviews
Easy to use

The reagent comes with a reaction buffer, which is convenient for enzyme digestion reactions.

United States

01/31/2022

High stability

The storage buffer for the enzyme contains BSA, which improves the stability of the enzyme. After being left at room temperature overnight, the enzyme activity is basically not affected.

United States

08/05/2022

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