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Esp3I

For research use only. Not intended for any clinical use.
Cat.No.
EROT0345
Reaction Buffer
33mM Tris-acetate (pH 7.9 at 37 °C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA,1.0 mM DTT
Reaction Conditions
Incubate at 37 °C
Recognition Site
CGTCTC(1/5)↓
Size/Form
1000 Units
Storage
10mM Tris-HCl (pH 7.5 at 25 °C), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.2mg/mL BSA and 50% (v/v) glycerol

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Q & A

Customer Reviews

Customer Q&As
What is the recognition enzyme site for Esp3I?

A: Esp3I recognizes the CGTCTC(1/5)^ site.

What class of product is Esp3I?

A: It is a restriction endonuclease.

Can Esp3I be used directly with the buffer provided?

A: No additional DTT is needed.

Is BSA needed to be added separately to the buffer?

A: No, Thermo Scientific restriction endonuclease reaction buffers include pre-mixed BSA, which enhances the stability of multiple enzymes and binds with contaminants present in the DNA preparation.

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