Hsp92I

Name: Hsp92I
SKU: EROT0375

For research use only. Not intended for any clinical use.

Cat.No.

EROT0375

Description

Haemophilus influenzae.

Concentration

10000 units/ml

Reaction Conditions

Buffer F. 37 °C.

Recognition Site

GR↓CGYC

Size/Form

500 units

Storage

10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Publications

Q & A

Customer Reviews

Customer Q&As

What is the optimal DNA concentration in restriction enzyme digestion?

A: The optimal range of DNA concentration is 0.02-0.1 μg/μL in the restriction digestion mixture. We would recommend the volume of the DNA sample not to exceed 30% of the total reaction volume to avoid potential reaction inhibition. To resolve inhibition from impurities of the DNA solution, we recommend increasing the overall volume of the reaction while keeping the volume of the DNA solution the same.

What are key factors promoting star activity?

A: Star activty may be contributed by prolonged incubation,high enzyme concentration,high glycerol concentration (usually 5% or higher) and small reaction volume.

Why do you recommend only 2 µL of 10X Reaction Buffer when digesting unpurified PCR product in a 30 µL reaction?

A: Because salts and ions from the PCR reaction would be carried over to the digestion reaction.

Is there any other way to inactivate Eco52I enzymes (when thermal inactivation is not applicable) without using phenol/chloroform?

A: Yes.Silica column based purification.

What are possible reasons for incomplete/failed restriction digestion?

A: The main reason for DNA cleavage reaction failure is the presence of contaminating inhibitors in the template DNA (for example: phenol, chloroform, detergents, ethanol, excess salts, EDTA, etc.).

Are there specific additives that can enhance the cleavage efficiency of Hsp92I in complex genomic structures?

A: It may require experimentation, such as trying the addition of different concentrations of DMSO or Betaine to enhance cleavage efficiency.

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