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PauI

For research use only. Not intended for any clinical use.
Cat.No.
EROT0431
Reaction Buffer
10mM Tris-HCl (pH 8.5 at 37 °C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
Reaction Conditions
Incubate at 37 °C
Recognition Site
G↓CGCGC
Size/Form
200 Units;400 Units
Storage
10mM Tris-HCl (pH 7.4 at 25°C), 100mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

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Q & A

Customer Reviews

Customer Q&As
PauI recognizes what site?

A: PauI restriction endonuclease recognizes the G^CGCGC site.

How stable is PauI during transportation if it undergoes freeze-thaw cycles?

A: During transportation for 24-48 hours, PauI can be transported on ice because its stability is not affected within a short period.

Does freezing PauI affect its performance?

A: PauI may freeze during transportation on dry ice, but this does not affect its stability, and it can still be used.

Is PauI sensitive to methylation?

A: PauI is sensitive to CpG methylation, insensitive to Dam methylation, and insensitive to Dcm methylation.

What is the optimal reaction temperature for PauI?

A: The optimal cutting temperature for PauI is 37°C.

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