Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : EROT0431
| Cat. No. | EROT0431 |
| Recognition Site | G↓CGCGC |
| Size | 200 Units;400 Units |
| Reaction Buffer | 10mM Tris-HCl (pH 8.5 at 37 °C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA |
| Reaction Conditions | Incubate at 37 °C |
| Storage | 10mM Tris-HCl (pH 7.4 at 25°C), 100mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol |
A: PauI restriction endonuclease recognizes the G^CGCGC site.
A: During transportation for 24-48 hours, PauI can be transported on ice because its stability is not affected within a short period.
A: PauI may freeze during transportation on dry ice, but this does not affect its stability, and it can still be used.
A: PauI is sensitive to CpG methylation, insensitive to Dam methylation, and insensitive to Dcm methylation.
A: The optimal cutting temperature for PauI is 37°C.
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