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PagI

For research use only. Not intended for any clinical use.
Cat.No.
EROT0429
Reaction Buffer
50mM Tris-HCl (pH 7.5 at 37 °C), 10mM MgCl2, 100mM NaCl and 0.1mg/mL BSA
Reaction Conditions
Incubate at 37 °C
Recognition Site
T↓CATGA
Size/Form
400 Units;800 Units
Storage
10mM Tris-HCl (pH 7.4 at 25°C), 100mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

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Q & A

Customer Reviews

Customer Q&As
Does PagI exhibit star activity?

A: PagI may exhibit star activity under low salt, high glycerol (>5%), pH > 8.0, or excess enzyme conditions.

Are there any other considerations for PagI?

A: Precautions for PagI include reduced activity when overlapping Dam methylation is present. To avoid Dam methylation, use dam- or dcm- strains, such as GM2163.

What is the optimal reaction temperature for PagI, and is it sensitive to heat inactivation?

A: PagI has good cutting efficiency at 37°C and is sensitive to heat inactivation, so it is best to incubate at 37°C.

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