Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : EROT0429
| Cat. No. | EROT0429 |
| Recognition Site | T↓CATGA |
| Size | 400 Units;800 Units |
| Reaction Buffer | 50mM Tris-HCl (pH 7.5 at 37 °C), 10mM MgCl2, 100mM NaCl and 0.1mg/mL BSA |
| Reaction Conditions | Incubate at 37 °C |
| Storage | 10mM Tris-HCl (pH 7.4 at 25°C), 100mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol |
A: PagI may exhibit star activity under low salt, high glycerol (>5%), pH > 8.0, or excess enzyme conditions.
A: Precautions for PagI include reduced activity when overlapping Dam methylation is present. To avoid Dam methylation, use dam- or dcm- strains, such as GM2163.
A: PagI has good cutting efficiency at 37°C and is sensitive to heat inactivation, so it is best to incubate at 37°C.
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