LguI

Name: LguI
SKU: EROT0380

For research use only. Not intended for any clinical use.

Cat.No.

EROT0380

Reaction Buffer

33mM Tris-acetate (pH 7.9 at 37 °C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA

Reaction Conditions

Incubate at 37 °C

Recognition Site

GCTCTTC(1/4)↓

Size/Form

500 Units

Storage

10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Publications

Q & A

Customer Reviews

Customer Q&As

How to determine the optimal cutting conditions for LguI restriction enzyme?

A: The optimal cutting conditions for LguI can be identified by testing different ion strengths, pH values, and temperatures. Manufacturers typically provide recommended reaction conditions.

What could cause incomplete digestion by LguI restriction enzyme?

A: Incomplete digestion may be due to poor DNA quality, DNA methylation, low enzyme activity, or inappropriate reaction buffer systems.

How to deal with the decrease in activity of LguI restriction enzyme after long-term storage?

A: The enzyme should be stored under the conditions recommended by the manufacturer and avoid repeated freeze-thaw cycles. If activity decreases, consider increasing the amount of enzyme used.

Can LguI restriction enzyme be used to construct recombinant vectors?

A: LguI can be used to construct recombinant vectors, but it is important to ensure that the enzyme cutting sites on the vector and the insert are matched and that the ends of the insert are compatible.

How to avoid self-ligation of DNA fragments when using LguI restriction enzyme?

A: Self-ligation can be prevented by dephosphorylating the 5'-phosphate groups of the fragments or by using the enzyme's star activity conditions.

Does LguI restriction enzyme require sequence specificity for DNA cleavage?

A: Yes, LguI recognizes and cuts specific nucleotide sequences, so it is essential to ensure that the target DNA contains the recognition sequence for LguI in the experimental design.

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