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CviJ I*

For research use only. Not intended for any clinical use.

Cat. No. :   EROT0322

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Product Information

Cat. No. EROT0322
Source Chlorella virus IL-3A
Recognition Site G↓C(*exceptPuG↓Cpy)
Size 25 Units;100 Units
Reaction Buffer 20 mM glycyl-glycine KOH (pH 8.5)10 mM magnesium acetate50 mM potassium acetate0.1 mM ATP0.1 mM dithiothreitolNote: 100% DMSO supplied separately
Reaction Conditions Incubation at 37 °C
Storage 20 mM Tris-acetate (pH 8.0 at 4 °C)0.5 mM EDTA0.1 mM dithiothreitol5 mM magnesium chloride50 mM potassium acetate50% (v/v) glycerol
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Customer Q&As
Can CviJ I* only cut single-stranded DNA?

A: No, both single-stranded and double-stranded DNA can be cleaved.

What are the heat inactivation conditions for CviJ I*?

A: 65°C for 10 minutes.

What is the product size of CviJ I* digestion?

A: Capable of cleaving single-stranded DNA and double-stranded DNA into small 20-200 bp fragments.

What are the reaction conditions for CviJ I*?

A: Incubation for 3 hours at 37°C in a total reaction volume of 25 μl.

Can CviJ I* be stored at -20°C?

A: Storage conditions should be at -80°C.

Can the reaction products of CviJ I* be used directly for electrophoresis?

A: CviJ I* reaction buffer contains DMSO, which does not interfere with further enzymatic manipulations (ligations, labeling, etc). If the sample is intended for electrophoresis, ethanol precipitation of the reaction mixture after completed digestion is strongly recommended to avoid diffused bands on agarose or polyacrylamide gels.

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