Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : EROT0322
| Cat. No. | EROT0322 |
| Source | Chlorella virus IL-3A |
| Recognition Site | G↓C(*exceptPuG↓Cpy) |
| Size | 25 Units;100 Units |
| Reaction Buffer | 20 mM glycyl-glycine KOH (pH 8.5)10 mM magnesium acetate50 mM potassium acetate0.1 mM ATP0.1 mM dithiothreitolNote: 100% DMSO supplied separately |
| Reaction Conditions | Incubation at 37 °C |
| Storage | 20 mM Tris-acetate (pH 8.0 at 4 °C)0.5 mM EDTA0.1 mM dithiothreitol5 mM magnesium chloride50 mM potassium acetate50% (v/v) glycerol |
A: No, both single-stranded and double-stranded DNA can be cleaved.
A: 65°C for 10 minutes.
A: Capable of cleaving single-stranded DNA and double-stranded DNA into small 20-200 bp fragments.
A: Incubation for 3 hours at 37°C in a total reaction volume of 25 μl.
A: Storage conditions should be at -80°C.
A: CviJ I* reaction buffer contains DMSO, which does not interfere with further enzymatic manipulations (ligations, labeling, etc). If the sample is intended for electrophoresis, ethanol precipitation of the reaction mixture after completed digestion is strongly recommended to avoid diffused bands on agarose or polyacrylamide gels.
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