Cat.No. : CSC-RR0256 Host Cell: A549
| Cat. No. | CSC-RR0256 |
| Description | Luc/GFP Reporter Cell Line-A549 is engineered to stably co-express GFP and luciferase reporter genes. It is an ideal positive control for use in bioluminescence and fluorescence assays. In addition, A549 cells can form tumors in mouse lungs. The in vivo growth of these tumors can be monitored using bioluminescent imaging. |
| Gene | Luciferase/GFP |
| Host Cell | A549 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Seeking efficient antiviral treatments, researchers concentrated on the difficulties presented by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The need for biosafety level 3 (BSL-3) confinement often limits traditional approaches. A noninfectious SARS-CoV-2 reporter replicon was built to solve this problem and offers a special forum for investigating viral replication processes and evaluating possible inhibitors. This replicon is competent for replication but does not produce infectious virions, making it a safe alternative for high-throughput antiviral assays. Notably, differences in inhibitor efficacy across various cell lines highlight the need for diverse experimental models, underscoring the importance of the A549 cell line in such studies.
Figure 1. The researchers utilized electroporation of A549 cells with an in vitro-transcribed SARS-CoV-2 replicon RNA to study viral replication and evaluate potential antiviral compounds. (He X, et al., 2021)
The Luc/GFP Reporter Cell Line-A549 from Creative Biogene offers researchers a powerful tool for investigating similar viral mechanisms and antiviral strategies. This cell line enables the expression of luciferase and GFP, allowing for real-time monitoring of viral replication and screening of therapeutic candidates.
A: The Luc/GFP Reporter Cell Line-A549, which expresses both luciferase and green fluorescent protein, can be exposed to different environmental stressors such as oxidative stress, heat shock, or chemical toxins. The changes in luciferase activity can be monitored to assess the activation of specific promoters, while GFP expression levels can indicate changes in cellular processes or stress responses, providing valuable insights into the transcriptional regulation mechanisms under stress conditions.
A: The cell line can be genetically modified to include reporters under the control of cytochrome P450 gene promoters. When exposed to xenobiotics, changes in luciferase and GFP expression can be correlated with the induction or inhibition of cytochrome P450 enzymes, which are crucial for drug metabolism. This approach allows for the identification of compounds that modulate drug-metabolizing enzymes and can be used to predict potential drug-drug interactions.
A: By introducing microRNA mimics or inhibitors into the Luc/GFP Reporter Cell Line-A549, researchers can study the effects of microRNAs on the expression of genes of interest. The cell line can be engineered to have luciferase or GFP reporters under the control of 3' UTR regions of target genes, allowing for the direct observation of microRNA-mediated gene regulation effects on transcription and translation.
A: The cell line can be used to test the efficiency of gene therapy vectors by incorporating therapeutic genes under the control of different promoters. By measuring the resulting luciferase and GFP expression levels, researchers can evaluate the effectiveness of the vectors in transducing the cells and the therapeutic gene's expression, which can inform the design of more efficient gene therapy strategies.
A: The dual-reporter system in the A549 cell line can be used to track cancer cell growth, survival, and invasiveness. By introducing oncogenes or tumor suppressor genes, researchers can monitor changes in luciferase and GFP activity to model tumor progression. Additionally, the cell line can be used to study the effects of potential anti-cancer drugs on these processes, providing a valuable tool for cancer therapy development and understanding the metastatic process.
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The Luc/GFP Reporter Cell Line-A549 is human-derived, making it highly relevant for translational research that aims to understand human cellular responses, particularly in the context of lung biology.
With both luciferase and GFP, the Luc/GFP Reporter Cell Line-A549 enables multiplexing capabilities, allowing simultaneous monitoring of multiple biological processes or pathways within the same cell population.
This cell line ensures stable expression of both reporters, providing consistent signals across passages and experiments, which is vital for the reproducibility of results in long-term studies.
The luciferase in the Luc/GFP Reporter Cell Line-A549 allows for chemiluminescent detection, offering high sensitivity and low background in detecting reporter activity, which is especially useful for assays with low signal strength.
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