Cat.No. : CSC-RR0368 Host Cell: C1498
| Cat. No. | CSC-RR0368 |
| Description | Luc/GFP Reporter Cell Line - C1498 is engineered to stably co-express GFP and luciferase reporter genes. It is an ideal positive control for use in bioluminescence and fluorescence assays. In addition, C1498 cells form tumors post implantation into immunocompromised mice. The in vivo growth of these tumors can be monitored using bioluminescent imaging. Tumor samples can also be collected postmortem for analysis by conventional fluorescence microscopy or flow cytometry. |
| Gene | Luciferase/GFP |
| Host Cell | C1498 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Luc/GFP Reporter Cell Line - C1498 provides a unique advantage by offering a dual-reporter system that allows for the simultaneous monitoring of two different biological processes. The luciferase (Luc) reporter gene is often used to measure gene expression levels, while the green fluorescent protein (GFP) serves as a marker for cellular localization or function. This dual-reporter system enables researchers to study the interplay between gene expression and cellular signaling pathways in real-time, providing a more comprehensive understanding of complex biological phenomena.
A: The Luc/GFP Reporter Cell Line - C1498 can serve as a valuable model for evaluating gene therapy approaches. By introducing therapeutic genes or siRNA constructs into these cells, researchers can monitor the effects on both gene expression (via Luc) and cellular function (via GFP). This dual monitoring allows for a more nuanced assessment of therapeutic efficacy, as it provides information on both the molecular and cellular levels of the therapy's impact.
A: For drug screening with the Luc/GFP Reporter Cell Line - C1498, it is critical to optimize conditions such as cell seeding density, incubation time, and drug exposure duration. Additionally, the choice of substrates for the luciferase assay and the methods for detecting GFP fluorescence must be carefully selected to ensure sensitivity and specificity. By standardizing these conditions, researchers can obtain reliable and reproducible data that can be used to identify potential drug candidates and evaluate their mechanisms of action.
A: The GFP component of the Luc/GFP Reporter Cell Line - C1498 enables researchers to visually track the movement and localization of proteins within the cell. By fusing the gene of interest to the GFP sequence, the dynamics of protein trafficking can be monitored in real-time, providing insights into the mechanisms of protein sorting and compartmentalization. This can be particularly useful for studying organelle-specific targeting signals and the effects of various cellular stresses on protein localization.
A: The Luc/GFP Reporter Cell Line - C1498 can be a powerful tool in systems biology, where the goal is to understand the complex interactions between various cellular components. By using this cell line in combination with high-throughput screening technologies and computational modeling, researchers can analyze the effects of genetic perturbations on both gene expression (Luc) and cellular function (GFP). This integrated approach can help construct detailed maps of cellular networks, revealing how different components interact and influence each other in response to various biological cues.
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The Luc/GFP Reporter Cell Line - C1498 combines luciferase and GFP, providing both luminescent and fluorescent reporting options, enhancing assay versatility.
Tailored for leukemia research, the Luc/GFP Reporter Cell Line - C1498 is a valuable tool for studying leukemia pathogenesis and testing treatments in a disease-relevant context.
GFP allows for real-time tracking in the Luc/GFP Reporter Cell Line - C1498, enabling the observation of leukemia cell dynamics and migration without disrupting cell viability.
The cell line produces quantitative bioluminescent data, crucial for assessing gene activity and cellular response with precision in the Luc/GFP Reporter Cell Line - C1498.
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