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Cat.No. : CSC-RR0238 Host Cell: CT26
| Cat. No. | CSC-RR0238 |
| Description | Luc/GFP Reporter Cell Line-CT26 is engineered to stably co-express GFP and luciferase reporter genes. It is an ideal positive control for use in bioluminescence and fluorescence assays. In addition, CT26 cells can form tumors post implantation into mice. The in vivo growth of these tumors can be monitored using bioluminescent imaging or optical imaging for GFP fluorescence. |
| Gene | Luciferase/GFP |
| Host Cell | CT26 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
High-dose radiation treatment may efficiently change the immunological microenvironment of mouse colon tumors, especially CT26 and MC38, producing complete remissions. Although the research reveals that a single radiation dosage of 30 Gy causes notable alterations, like enhanced CD8+ T cell infiltration and reduced myeloid-derived suppressor cells (MDSCs), these tumors are poorly immunogenic, which presents obstacles to therapy. The procedure generates a strong anti-tumor immune response driven by IFN-γ using antigen cross-presentation by CD8+ dendritic cells and the activity of CD4+ T cells.
Figure 1. The researchers transduced CT26 cells with a GFP-firefly luciferase fusion gene, enhancing transduction efficiency to create a stable cell line for tumor immune studies. (Filatenkov A, et al., 2015)
Creative Biogene's Luc/GFP Reporter Cell Line-CT26 product is designed for researchers exploring similar immune response dynamics in tumor studies. This stable cell line, engineered for bioluminescence and fluorescence, allows for real-time monitoring of tumor behavior and immune interactions in vivo.
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In the process of obtaining data using the Luc/GFP Reporter Cell Line-CT26 product, I found the results to be very reliable. This cell line has demonstrated good reproducibility and consistency, both within the lab and in communication with other research teams. This gives me confidence in the results of my experiments and facilitates collaboration and communication with others.
United States
09/18/2022
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