Cat.No. : CSC-RR0360 Host Cell: NALM6
| Cat. No. | CSC-RR0360 |
| Description | Luc/GFP Reporter Cell Line - NALM6 is engineered to stably co-express GFP and luciferase reporter genes. It is an ideal positive control for use in bioluminescence and fluorescence assays. In addition, NALM6 cells form tumors post implantation into immunocompromised mice. The in vivo growth of these tumors can be monitored using bioluminescent imaging. Tumor samples can also be collected postmortem for analysis by conventional fluorescence microscopy or flow cytometry. |
| Gene | Luciferase/GFP |
| Host Cell | NALM6 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Despite CAR T-cell therapy's success against hematologic malignancies, resistance and recurrence persist, with unclear underlying markers or mechanisms. Researchers conducted an unbiased genome-wide CRISPR/Cas9 screening to investigate mechanisms of resistance to CAR T-cell therapy in hematologic malignancies. They identified loss of NOXA, a BCL2 family protein, as a critical regulator of resistance. Low NOXA expression correlated with worse survival in clinical trials. Pharmacological enhancement of NOXA sensitized cancer cells to CAR T-cell therapy both in vitro and in vivo. This study highlights NOXA as a potential predictive marker for CAR T-cell therapy response and survival, suggesting pharmacological targeting of NOXA as a promising strategy to enhance CAR T-cell therapy efficacy.
Figure 1. The Luc Reporter Cell Line - NALM6, engineered to express GFP and firefly luciferase, was utilized by researchers for bioluminescence imaging studies. NALM6 cells were infected with lentivirus constructs to express GFP virus and firefly luciferase, with mCherry also introduced for enhanced imaging capabilities. (Yan X, et al., 2022)
A: Luc/GFP Reporter Cell Line - NALM6 provides a dual-reporter system for comprehensive analysis of gene expression and cellular responses in leukemia cells. The luciferase reporter enables real-time monitoring of transcriptional activity, while the GFP reporter allows for visualization and tracking of cell populations, facilitating detailed studies of leukemia progression and treatment response.
A: Luc/GFP Reporter Cell Line - NALM6 undergoes stringent validation to ensure stable expression of both luciferase and GFP reporters. Our standardized protocols for stable transfection and clonal selection guarantee consistent reporter expression levels, ensuring reliable and reproducible data in leukemia studies.
A: NALM6 cells were likely selected for their relevance to leukemia research and their amenability to stable transgene expression. The combination of luciferase and GFP reporters allows for the investigation of gene expression, cellular processes, and leukemia progression using bioluminescence imaging and fluorescence microscopy.
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Enhanced insights! Its dual-reporter system provides multifaceted data, empowering deeper understanding of leukemia pathogenesis and therapeutic targets.
The Luc/GFP Reporter Cell Line in NALM6 cells offers a powerful combination of luciferase and GFP, enabling comprehensive analysis of gene expression and cellular processes.
The Luc/GFP Reporter Cell Line simplifies experimental workflows, allowing for efficient data collection and analysis in leukemia research. From studying leukemic cell behavior to evaluating treatment efficacy, this cell line serves as an invaluable resource for unraveling the complexities of leukemia biology and advancing novel therapies.
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