Human PTEN Knockout Cell Line-Panc-1

Name: Human PTEN Knockout Cell Line-Panc-1
SKU: CSC-RT2664
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2664

Target Gene : PTEN Host Cell : PANC-1

Size : >1x10⁶ cells/vial Validation : Sequencing

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2664
Description	This cell is a stable cell line with a homozygous knockout of human PTEN using CRISPR/Cas9.
Target Gene	PTEN
Host Cell	PANC-1
Host Cell Species	Homo sapiens (Human)
Size	1 vial (10^6 cell/vial)
Validation	Sequencing
Storage	Liquid nirtogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PTEN phosphatase and tensin homolog [ Homo sapiens ]
Gene Symbol	PTEN
Synonyms	BZS; DEC; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del
Gene Description	phosphatase and tensin homolog (mutated in multiple advanced cancers 1)
Gene ID	5728
Uni Prot ID	F6KD01
m RNA Refseq	NM_000314.4
Protein Refseq	NP_000305.3
Chromosome Location	10q23.3
Function	PDZ domain binding; anaphase-promoting complex binding; enzyme binding; inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity; lipid binding; magnesium ion binding; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity; phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity; phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity; phosphatidylinositol-3-phosphatase activity; phosphoprotein phosphatase activity; protein binding; protein kinase binding; protein serine/threonine phosphatase activity; protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity;
Pathway	3-phosphoinositide degradation, organism-specific biosystem; 3-phosphoinositide degradation, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; BCR signaling pathway, organism-specific biosystem; Class I PI3K signaling events, organism-specific biosystem; Constitutive PI3K/AKT Signaling in Cancer, organism-specific biosystem;
MIM	601728

Quick Inquiry

Background

Applications

Q & A

Customer Reviews

PTEN (phosphatase and tensin homolog) is an important tumor suppressor gene that plays an important role in regulating the cell cycle and preventing cells from growing and dividing too rapidly or uncontrollably. PTEN was first discovered in 1997 and is located on chromosome 10 (10q23), and is known for encoding a protein with dual-specificity phosphatase activity. This activity enables it to remove phosphate groups from proteins and lipids, specifically targeting the lipid second messenger phosphatidylinositol (3,4,5)-triphosphate (PIP3), thereby acting as a negative regulator of the PI3K/AKT signaling pathway.

PTEN gene mutations or deletions are commonly found in various types of cancer, including breast cancer, prostate cancer, endometrial cancer, glioblastoma, etc. Loss of PTEN function can lead to uncontrolled cell growth and survival, significantly promoting tumor development and progression. Furthermore, germline mutations in the PTEN gene have been associated with several inherited disorders, collectively known as PTEN hamartoma syndrome (PHTS), including Cowden syndrome, Bannayan-Riley-Ruvalcaba syndrome, and Proteus syndrome. In addition to its known role in cancer suppression, recent studies have uncovered broader effects of PTEN in other biological processes, such as metabolism, aging, and neurodevelopment.

The Human PTEN Knockout Cell Line (Panc-1) is a crucial tool in biomedical research, primarily used to study the role of PTEN (Phosphatase and Tensin Homolog) in cancer biology, especially in pancreatic cancer. Here are some key applications:

Cancer Research: The PTEN Knockout Cell Line-Panc-1 is extensively used in cancer research to study the role of the PTEN gene in tumorigenesis. PTEN is a well-known tumor suppressor, and its knockout in Panc-1 cells helps investigate the molecular mechanisms of pancreatic cancer progression and resistance to therapy.

Drug Testing: This cell line is instrumental in drug discovery and development. By using PTEN-deficient Panc-1 cells, researchers can screen potential therapeutic compounds that could target the pathways altered due to loss of PTEN, potentially leading to new cancer treatments.

Signal Transduction Studies: PTEN is a critical regulator of the PI3K/AKT signaling pathway. Researchers utilize the PTEN Knockout Cell Line-Panc-1 to explore alterations in cell signaling events, aiding in the understanding of how PTEN loss affects various cellular processes, including growth, survival, and metabolism.

Genetic Research: This cell line serves as a valuable model for genetic studies focused on gene function and interaction. It provides insights into the consequences of PTEN deletion at a genetic level, contributing to broader knowledge about gene networks and regulatory mechanisms in human cells.

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Customer Reviews

High-quality cells

The quality of the cells is outstanding, and they have shown remarkable consistency across multiple experiments. This has enabled us to generate reproducible and reliable data, significantly accelerating our study on cancer pathways.

United Kingdom

2023-11-24

Streamlined our research process

This PTEN knockout Panc-1 cell line has streamlined our workflow. We no longer need to spend valuable time and resources on gene editing and validation.

United States

2020-05-29

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *

Name

Country *

Email *

Related Products

Cat.No.	Product Name	Price
CSC-RK0062	Nos3 Knockout Mouse Liver Cells	Inquiry
CSC-RK0077	Tlr4 Knockout Mouse Embryonic Stem Cells	Inquiry
CSC-RK0114	Cybb Knockout Mouse Heart Cells	Inquiry
CSC-RK0076	Tlr4 Knockout Mouse Embryonic Fibroblasts	Inquiry
CSC-RK0058	Nos3 Knockout Mouse Embryonic Stem Cells	Inquiry
CSC-RK0110	Cybb Knockout Mouse Bone Marrow Neutrophils	Inquiry
CSC-RK0053	Nos3 Knockout Mouse Lung Fibroblasts	Inquiry
CSC-RK0112	Cybb Knockout Mouse Embryonic Stem Cells	Inquiry
CSC-RK0064	Nos3 Knockout Mouse Lung Cells	Inquiry
CSC-RK0081	Tlr4 Knockout Mouse Kidney Cells	Inquiry

Human PTEN Knockout Cell Line-Panc-1

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Applications

Q & A

Customer Reviews

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

High-quality cells

Streamlined our research process

Related Products

Related Services