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Human PTEN Knockout Cell Line-MCF10A

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0032

Target Gene :   PTEN Host Cell :   MCF10A

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT0032
Description MCF10A-PTEN (-/-) is a cell line with a homozygous knockout of human PTEN
Target Gene PTEN
Host Cell MCF10A
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name PTEN phosphatase and tensin homolog [ Homo sapiens ]
Gene Symbol PTEN
Synonyms BZS; DEC; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del
Gene Description phosphatase and tensin homolog (mutated in multiple advanced cancers 1)
Gene ID 5728
Uni Prot ID F6KD01
m RNA Refseq NM_000314.4
Protein Refseq NP_000305.3
Chromosome Location 10q23.3
Function PDZ domain binding; anaphase-promoting complex binding; enzyme binding; inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity; lipid binding; magnesium ion binding; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity; phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity; phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity; phosphatidylinositol-3-phosphatase activity; phosphoprotein phosphatase activity; protein binding; protein kinase binding; protein serine/threonine phosphatase activity; protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity;
Pathway 3-phosphoinositide degradation, organism-specific biosystem; 3-phosphoinositide degradation, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; BCR signaling pathway, organism-specific biosystem; Class I PI3K signaling events, organism-specific biosystem; Constitutive PI3K/AKT Signaling in Cancer, organism-specific biosystem;
MIM 601728
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Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Positive results

Our lab ordered three knockout cell lines at the same time, I used this knockout cell line first and the transfection efficiency was good, the students who used other transfection cell lines also reported good results.

United States

Valid for testing

First time using transfection products, I have ordered gene fragments before and felt that the results were not very good, this transfection cell line is good, the results are good and can be tested in multiple ways.

United States

Save money

I think this transfection cell line is not bad, I used to order a foreign brand, now I think I can use this transfection cell line for a long time and save money.

United States

Convincing results

The most fearful thing is to take a wrong turn in choosing the cell lines for the experiment. I spent two months before doing the experiment without positive results and was so anxious. Now, using this cell line, you can see the results of the original concept little by little.

United States

High efficiency

This cell line may be a little less expensive than foreign cell lines, but for its price, I think it is the most efficient cell line in its class.

United States

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