Cat.No. : CSC-DC012651
Host Cell: HEK293 (Hela and other cell types are also available) Validation: Real-Time RCR
| Cat. No. | CSC-DC012651 |
| Description | Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free. |
| Gene | PTEN |
| Host Cell | HEK293 (Hela and other cell types are also available) |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | (1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Size Form | >1 × 10^6 cells / vial |
| Shipping | Dry Ice |
| Storage | Liquid Nitrogen |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: PTEN gene (phosphate and tension homolog deleted on chromosome 10, PTEN), also known as MMAC1 (mutated in multiple advanced cancers1) and TEP1 (TGF-regulated and epithelial cell-enriched phosphatase).
A: PTEN proteins inhibit tumour development by antagonising the activity of phosphatases such as tyrosine kinases. It was found to have a significant inhibitory effect on the activity of tyrosine kinase FAK in tumour cells. PTEN proteins play a role in cell growth signalling pathways.
A: PTEN is localized on chromosome 10q23.3 and consists of nine exons encoding a protein consisting of 403 amino acids with phosphodiesterase activity.
A: PTEN protein inhibits tumor development by antagonizing the activity of phosphatases such as tyrosine kinase.
A: wild-type PTEN gene transfection into glioblastoma with abnormalities of this gene, the growth and invasive ability of tumor cells were significantly inhibited, and the activity of tyrosine kinase FAK of tumor cells was found to be significantly inhibited.
A: The PTEN gene regulates the synthesis of a specific protein that is contained in almost all tissues in the body.
A: This protein acts as a tumor suppressor and in turn regulates the cell division cycle by preventing cells from growing, dividing too fast or dividing uncontrollably.
A: The phosphate group consists of three oxygen atoms and one phosphorus atom, and PTEN proteins can modify other proteins and lipids by removing the phosphate group. Based on this property, PTEN proteins are characterized as phosphatases.
A: PTEN proteins have important roles in cell growth, apoptosis, adhesion, migration, and infiltration.
A: The phosphatase functional region of PTEN includes the (I/V)-H-C-X-A-G-X-X-R-(S/T)-G module, which is also present in tyrosine and dual-specificity (dual-specifity) phosphatases.
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