Panoply™ Human PTEN Knockdown Stable Cell Line

Name: Panoply™ Human PTEN Knockdown Stable Cell Line
SKU: CSC-DC012651
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-DC012651

Host Cell : HEK293 (Hela and other cell types are also available) Validation : Real-Time RCR

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Cell Line Information

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Gene Information

Cat. No.	CSC-DC012651
Description	Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Target Gene	PTEN
Host Cell	HEK293 (Hela and other cell types are also available)
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid Nitrogen
Shipping	Dry Ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PTEN phosphatase and tensin homolog [ Homo sapiens ]
Gene Symbol	PTEN
Synonyms	BZS; DEC; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del
Gene Description	phosphatase and tensin homolog (mutated in multiple advanced cancers 1)
Gene ID	5728
Uni Prot ID	F6KD01
m RNA Refseq	NM_000314.4
Protein Refseq	NP_000305.3
Chromosome Location	10q23.3
Function	PDZ domain binding; anaphase-promoting complex binding; enzyme binding; inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity; lipid binding; magnesium ion binding; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity; phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity; phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity; phosphatidylinositol-3-phosphatase activity; phosphoprotein phosphatase activity; protein binding; protein kinase binding; protein serine/threonine phosphatase activity; protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity;
Pathway	3-phosphoinositide degradation, organism-specific biosystem; 3-phosphoinositide degradation, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; BCR signaling pathway, organism-specific biosystem; Class I PI3K signaling events, organism-specific biosystem; Constitutive PI3K/AKT Signaling in Cancer, organism-specific biosystem;
MIM	601728

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Q & A

Customer Reviews

Customer Q&As

What is PTEN？

A: PTEN gene (phosphate and tension homolog deleted on chromosome 10, PTEN), also known as MMAC1 (mutated in multiple advanced cancers1) and TEP1 (TGF-regulated and epithelial cell-enriched phosphatase).

What is the role of PTEN?

A: PTEN proteins inhibit tumour development by antagonising the activity of phosphatases such as tyrosine kinases. It was found to have a significant inhibitory effect on the activity of tyrosine kinase FAK in tumour cells. PTEN proteins play a role in cell growth signalling pathways.

What is the localization of PTEN on the chromosome?

A: PTEN is localized on chromosome 10q23.3 and consists of nine exons encoding a protein consisting of 403 amino acids with phosphodiesterase activity.

How does PTEN protein suppress tumors?

A: PTEN protein inhibits tumor development by antagonizing the activity of phosphatases such as tyrosine kinase.

What is the mechanism of action of PTEN in tumor suppression?

A: wild-type PTEN gene transfection into glioblastoma with abnormalities of this gene, the growth and invasive ability of tumor cells were significantly inhibited, and the activity of tyrosine kinase FAK of tumor cells was found to be significantly inhibited.

What is the basis for the PTEN gene being used as a phosphatase?

A: The PTEN gene regulates the synthesis of a specific protein that is contained in almost all tissues in the body.

How do PTEN genes regulate the cell division cycle?

A: This protein acts as a tumor suppressor and in turn regulates the cell division cycle by preventing cells from growing, dividing too fast or dividing uncontrollably.

Why PTEN proteins are characterized as phosphatases？

A: The phosphate group consists of three oxygen atoms and one phosphorus atom, and PTEN proteins can modify other proteins and lipids by removing the phosphate group. Based on this property, PTEN proteins are characterized as phosphatases.

Why is there an important role for PTEN proteins?

A: PTEN proteins have important roles in cell growth, apoptosis, adhesion, migration, and infiltration.

What parts of the phosphatase functional region of PTEN are included?

A: The phosphatase functional region of PTEN includes the (I/V)-H-C-X-A-G-X-X-R-(S/T)-G module, which is also present in tyrosine and dual-specificity (dual-specifity) phosphatases.

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