Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT0007
Target Gene : PTEN Host Cell : DLD-1
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT0007 |
| Description | DLD-1-PTEN (-/-) is a cell line with a homozygous knockout of human PTEN |
| Target Gene | PTEN |
| Host Cell | DLD-1 |
| Host Cell Species | Homo sapiens (Human) |
| Size | >1x106 cells/vial |
| Validation | Sequencing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | PTEN phosphatase and tensin homolog [ Homo sapiens ] |
| Gene Symbol | PTEN |
| Synonyms | BZS; DEC; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del |
| Gene Description | phosphatase and tensin homolog (mutated in multiple advanced cancers 1) |
| Gene ID | 5728 |
| Uni Prot ID | F6KD01 |
| m RNA Refseq | NM_000314.4 |
| Protein Refseq | NP_000305.3 |
| Chromosome Location | 10q23.3 |
| Function | PDZ domain binding; anaphase-promoting complex binding; enzyme binding; inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity; lipid binding; magnesium ion binding; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity; phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity; phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity; phosphatidylinositol-3-phosphatase activity; phosphoprotein phosphatase activity; protein binding; protein kinase binding; protein serine/threonine phosphatase activity; protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; |
| Pathway | 3-phosphoinositide degradation, organism-specific biosystem; 3-phosphoinositide degradation, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; BCR signaling pathway, organism-specific biosystem; Class I PI3K signaling events, organism-specific biosystem; Constitutive PI3K/AKT Signaling in Cancer, organism-specific biosystem; |
| MIM | 601728 |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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