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Human PTEN Knockout Cell Line-DLD-1

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0007

Target Gene :   PTEN Host Cell :   DLD-1

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT0007
Description DLD-1-PTEN (-/-) is a cell line with a homozygous knockout of human PTEN
Target Gene PTEN
Host Cell DLD-1
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name PTEN phosphatase and tensin homolog [ Homo sapiens ]
Gene Symbol PTEN
Synonyms BZS; DEC; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del
Gene Description phosphatase and tensin homolog (mutated in multiple advanced cancers 1)
Gene ID 5728
Uni Prot ID F6KD01
m RNA Refseq NM_000314.4
Protein Refseq NP_000305.3
Chromosome Location 10q23.3
Function PDZ domain binding; anaphase-promoting complex binding; enzyme binding; inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity; lipid binding; magnesium ion binding; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity; phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity; phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity; phosphatidylinositol-3-phosphatase activity; phosphoprotein phosphatase activity; protein binding; protein kinase binding; protein serine/threonine phosphatase activity; protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity;
Pathway 3-phosphoinositide degradation, organism-specific biosystem; 3-phosphoinositide degradation, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; BCR signaling pathway, organism-specific biosystem; Class I PI3K signaling events, organism-specific biosystem; Constitutive PI3K/AKT Signaling in Cancer, organism-specific biosystem;
MIM 601728
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Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Costly

The price of such a stable and effective reagent is so affordable, I have told other research colleagues about the beauty of this product.

United States

Highly efficient

I saw the beauty of this reagent from the first experiment, and I was completely impressed by its high efficiency.

United States

Good effect

I blamed my experimental technique for the previous unsuccessful transfection, until I used this reagent and felt that I should reconsider the reason for the previous failure.

United States

Successful transfection

If you want stable experimental results, efficient knockout technology, and cheap price, then this reagent is undoubtedly the best for you.

United States

Good result

The next time you encounter experimental failure, don't be anxious all the time, maybe changing a brand of reagent will have different gains.

United States

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