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Cat.No. : CSC-RT0007
Host Cell: DLD-1 Target Gene: PTEN
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0007 |
| Description | DLD-1-PTEN (-/-) is a cell line with a homozygous knockout of human PTEN |
| Target Gene | PTEN |
| Host Cell | DLD-1 |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The price of such a stable and effective reagent is so affordable, I have told other research colleagues about the beauty of this product.
United States
12/02/2021
I saw the beauty of this reagent from the first experiment, and I was completely impressed by its high efficiency.
United States
02/06/2022
I blamed my experimental technique for the previous unsuccessful transfection, until I used this reagent and felt that I should reconsider the reason for the previous failure.
United States
09/10/2022
If you want stable experimental results, efficient knockout technology, and cheap price, then this reagent is undoubtedly the best for you.
United States
01/18/2023
The next time you encounter experimental failure, don't be anxious all the time, maybe changing a brand of reagent will have different gains.
United States
02/21/2023
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