Cat.No. : CSC-RO0670
| Cat. No. | CSC-RO0670 |
| Description | This cell line is engineered to stably overexpress human PDCD1 in a human CD274 knockout cell line. |
| Gene | PDCD1 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The PDCD1 gene encodes for the PD-L1 (programmed death-like 1) protein, which is a member of the B7 family of immune-regulatory proteins. PD-L1 is expressed on the surface of various cell types, including tumor cells and activated immune cells. It functions as a ligand for the PD-1 (programmed death 1) receptor, which is expressed on activated T cells and NK cells. The interaction between PD-L1 and PD-1 plays a key role in regulating immune responses and can promote tolerance or immune evasion in certain cancers.
A: The PDCD1 gene contributes to immune evasion in cancer by enabling tumor cells to express high levels of PD-L1. This overexpression can lead to the inhibition of T cells and NK cells, preventing them from effectively attacking and destroying tumor cells. This immune evasion mechanism allows cancer cells to survive and proliferate, leading to tumor progression and metastasis.
A: Targeting the PDCD1 gene and its protein product has significant therapeutic implications for the treatment of various cancers. Immunotherapies that block the interaction between PD-L1 and PD-1, such as antibodies against PD-L1 or PD-1, can enhance T cell activity and restore immune surveillance against cancer cells. These therapies have shown promising results in the treatment of melanoma, lung cancer, and other malignancies.
A: The expression of the PDCD1 gene is regulated by various factors, including cytokines, growth factors, and oncogenic signaling. In normal cells, PD-L1 expression is typically low. However, in cancerous cells, PDCD1 can become overexpressed due to genetic alterations or epigenetic changes, leading to increased PD-L1 production and contributing to immune evasion.
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The Human CD274(-)/PDCD1 Stable Cell Line demonstrates high adaptability, allowing for easy integration into various experimental setups and research protocols, making it a versatile tool for studying immune regulation and cancer immunotherapy.
Detailed technical documentation is provided with the Human CD274(-)/PDCD1 Stable Cell Line, including comprehensive information on cell line generation, characterization, and recommended culture conditions.
The Human CD274(-)/PDCD1 Stable Cell Line is designed to be portable and compatible with different laboratory environments, allowing researchers to easily transfer and establish the cell line in their own facilities without compromising its functionality or stability.
The provider offers excellent training and support to researchers working with the Human CD274(-)/PDCD1 Stable Cell Line, including guidance on cell culture techniques, experimental design, and data analysis. This ensures that researchers have the necessary skills and knowledge to effectively utilize the cell line in their research.
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