Cat.No. : CSC-RO0208 Host Cell: CHO-K1
| Cat. No. | CSC-RO0208 |
| Description | This cell line is engineered to stably overexpress the monkey PDCD1 in CHO-K1 cells. |
| Introduction | This cell line is constructed by transfection of monkey programmed cell death 1 (PDCD1) into CHO-K1, followed by stable cell selection. The expression of PDCD1 has been analyzed through flow cytometry. |
| Gene | PDCD1 |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Monkey PDCD1 Stable Cell Line - CHO-K1 is genetically engineered to express the PDCD1 (Programmed cell death protein 1) receptor, which is a critical immune checkpoint molecule. This modification allows the cell line to be used in studying the PD-1 pathway, which plays a significant role in T cell exhaustion and immune evasion in cancer and infectious diseases.
A: The Monkey PDCD1 Stable Cell Line - CHO-K1 provides a platform for high-throughput screening of compounds that modulate PD-1 activity. This can lead to the discovery of new therapeutic agents that enhance the immune response against cancer cells or pathogens, potentially leading to more effective immunotherapies.
A: By providing a controlled environment to study PD-1 expression and function, the Monkey PDCD1 Stable Cell Line - CHO-K1 can help researchers investigate the mechanisms by which PD-1 contributes to immune tolerance and its dysregulation in autoimmune diseases, potentially leading to new therapeutic targets.
A: The Monkey PDCD1 Stable Cell Line - CHO-K1 can be employed in high-throughput screening assays to identify patient-specific biomarkers and potential therapeutic agents that modulate PD-1 activity, contributing to the development of personalized treatment plans.
A: Long-term culture of the Monkey PDCD1 Stable Cell Line - CHO-K1 may be associated with challenges such as genetic drift, loss of protein expression, and potential contamination. Regular monitoring and quality control measures, including testing for mycoplasma contamination and verifying PDCD1 expression, are essential to maintain the cell line's integrity.
A: The Monkey PDCD1 Stable Cell Line - CHO-K1 provides a platform to study the interactions between PD-1 and immune cells, which can help researchers understand the role of PD-1 in immune evasion and the development of immune-based cancer therapies.
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