Monkey PDCD1 Stable Cell Line - CHO-K1

Name: Monkey PDCD1 Stable Cell Line - CHO-K1
SKU: CSC-RO0208
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0208

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0208
Description	This cell line is engineered to stably overexpress the monkey PDCD1 in CHO-K1 cells.
Introduction	This cell line is constructed by transfection of monkey programmed cell death 1 (PDCD1) into CHO-K1, followed by stable cell selection. The expression of PDCD1 has been analyzed through flow cytometry.
Target Gene	PDCD1
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Q & A

Customer Reviews

Customer Q&As

What is the genetic modification that characterizes the Monkey PDCD1 Stable Cell Line - CHO-K1, and how does it influence the cell's immune response?

A: The Monkey PDCD1 Stable Cell Line - CHO-K1 is genetically engineered to express the PDCD1 (Programmed cell death protein 1) receptor, which is a critical immune checkpoint molecule. This modification allows the cell line to be used in studying the PD-1 pathway, which plays a significant role in T cell exhaustion and immune evasion in cancer and infectious diseases.

How does the Monkey PDCD1 Stable Cell Line - CHO-K1 contribute to the development of immunotherapies targeting PD-1?

A: The Monkey PDCD1 Stable Cell Line - CHO-K1 provides a platform for high-throughput screening of compounds that modulate PD-1 activity. This can lead to the discovery of new therapeutic agents that enhance the immune response against cancer cells or pathogens, potentially leading to more effective immunotherapies.

How does the Monkey PDCD1 Stable Cell Line - CHO-K1 support the study of PD-1's role in autoimmune diseases?

A: By providing a controlled environment to study PD-1 expression and function, the Monkey PDCD1 Stable Cell Line - CHO-K1 can help researchers investigate the mechanisms by which PD-1 contributes to immune tolerance and its dysregulation in autoimmune diseases, potentially leading to new therapeutic targets.

How can the Monkey PDCD1 Stable Cell Line - CHO-K1 be used in the development of personalized medicine strategies targeting PD-1?

A: The Monkey PDCD1 Stable Cell Line - CHO-K1 can be employed in high-throughput screening assays to identify patient-specific biomarkers and potential therapeutic agents that modulate PD-1 activity, contributing to the development of personalized treatment plans.

What are the challenges associated with maintaining the Monkey PDCD1 Stable Cell Line - CHO-K1 in long-term culture, and how can these be mitigated?

A: Long-term culture of the Monkey PDCD1 Stable Cell Line - CHO-K1 may be associated with challenges such as genetic drift, loss of protein expression, and potential contamination. Regular monitoring and quality control measures, including testing for mycoplasma contamination and verifying PDCD1 expression, are essential to maintain the cell line's integrity.

How does the Monkey PDCD1 Stable Cell Line - CHO-K1 contribute to the understanding of PD-1's role in immune cell interactions?

A: The Monkey PDCD1 Stable Cell Line - CHO-K1 provides a platform to study the interactions between PD-1 and immune cells, which can help researchers understand the role of PD-1 in immune evasion and the development of immune-based cancer therapies.

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