Monkey PDCD1 Stable Cell Line - HEK293T

The PDCD1 gene encodes the programmed cell death protein 1 (PD-1), an immune checkpoint receptor belonging to the immunoglobulin superfamily that is predominantly expressed on the surface of activated T cells, B cells, and natural killer cells. As a key negative regulator of immune responses, PD-1 binds to its ligands PD-L1 and PD-L2 to suppress T cell activation and maintain peripheral immune tolerance. The Monkey PDCD1 Stable Cell Line - HEK293T is generated by transfecting the full-length cynomolgus monkey PDCD1 gene into HEK293T cells, followed by antibiotic selection to establish a homogeneous population that stably and constitutively expresses the monkey PD-1 protein on the cell membrane. This cell line provides a reliable and reproducible in vitro tool for studying the PD-1/PD-L1 signaling axis in a non-human primate context, which is particularly valuable given the high sequence homology between monkey and human PD-1.

This stable cell line is widely used in immuno-oncology research, especially for the screening and characterization of anti-PD-1 therapeutic antibodies and small molecule inhibitors during preclinical drug development. Researchers can employ this cell line in binding assays to evaluate the affinity and specificity of candidate antibodies targeting monkey PD-1, as well as in functional assays such as reporter gene systems to assess the blocking activity of immune checkpoint inhibitors. The HEK293T background offers high transfection efficiency and robust protein expression, making it suitable for flow cytometry, ELISA, and cell-based binding studies. Additionally, the line can be used to study receptor internalization, downstream signaling events, and cross-reactivity profiling between species. By providing a consistent and stable source of monkey PD-1-expressing cells, this product supports translational research aimed at bridging preclinical animal studies with clinical applications in human immunotherapy.