Cat.No. : CSC-RO0847 Host Cell: M-07e
| Cat. No. | CSC-RO0847 |
| Description | This cell line is engineered to stably overexpress human PDCD1 (also named as PD1). |
| Gene | PDCD1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | M-07e |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The stable expression of PDCD1 in the Human PDCD1 Stable Cell Line - M-07e is achieved by introducing the PDCD1 gene into M-07e cells using genetic engineering techniques. This typically involves cloning the PDCD1 gene into a suitable expression vector and then transfecting the vector into M-07e cells. Successfully transfected cells are then selected to form a cell line that stably expresses PDCD1.
A: The role of PDCD1 in tumor immunotherapy can be investigated by modulating its expression levels in the Human PDCD1 Stable Cell Line - M-07e. For example, RNA interference or CRISPR/Cas9 technology can be used to knock down or knockout the PDCD1 gene, and observe changes in tumor cell growth, migration, and invasion abilities. In addition, PDCD1 can be expressed in various types of tumor cells to study its functions in tumor immunotherapy.
A: The Human PDCD1 Stable Cell Line - M-07e can be used for screening potential drugs targeting PDCD1. For example, high-throughput screening methods can be employed to identify small molecule compounds that can inhibit PDCD1 activity or expression. These compounds can then be further studied for their antitumor activities, providing a foundation for the development of novel antitumor drugs.
A: To ensure the stability and activity of the Human PDCD1 Stable Cell Line - M-07e during experiments, strict aseptic techniques and appropriate culture conditions, such as temperature, humidity, and CO2 concentration, should be followed. Regular testing of the cells should be conducted to ensure they are free from contamination or genetic drift. In addition, avoid excessive passaging of the cells to maintain their stability.
A: The advantages of using the Human PDCD1 Stable Cell Line - M-07e to study the interaction between PDCD1 and other signaling pathways include: 1) stable expression of PDCD1, which facilitates the investigation of its role in signaling pathways; 2) the ability to observe the effects of PDCD1 on signaling pathways at the cellular level, revealing its functions in biological processes; 3) the possibility to further study the interaction between PDCD1 and other members of signaling pathways using gene editing technologies in this cell line.
A: When selecting control groups for experiments using the Human PDCD1 Stable Cell Line - M-07e, consider the following strategies: 1) use untransfected M-07e cells as controls to exclude the effects of other factors on the experimental results; 2) use M-07e cells transfected with an empty vector as controls to evaluate the contribution of PDCD1 expression to the results; 3) if studying the role of PDCD1 under specific conditions, other stable cell lines with similar characteristics can be used as controls.
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