Human PDCD1 Stable Cell Line - M-07e

Name: Human PDCD1 Stable Cell Line - M-07e
SKU: CSC-RO0847
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0847

Host Cell : M-07e Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0847
Description	This cell line is engineered to stably overexpress human PDCD1 (also named as PD1).
Target Gene	PDCD1
Gene Species	Homo sapiens (Human)
Host Cell	M-07e
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PDCD1 programmed cell death 1 [ Homo sapiens ]
Gene Symbol	PDCD1
Synonyms	PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l
Gene ID	5133
Uni Prot ID	Q15116
m RNA Refseq	NM_005018.2
Protein Refseq	NP_005009.2
Chromosome Location	2q37.3
Function	protein binding; signal transducer activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Immune System, organism-specific biosystem; PD-1 signaling, organism-specific biosystem; T cell receptor signaling pathway, organism-specific biosystem;
MIM	600244

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the stable expression of PDCD1 achieved in the Human PDCD1 Stable Cell Line - M-07e through genetic engineering?

A: The stable expression of PDCD1 in the Human PDCD1 Stable Cell Line - M-07e is achieved by introducing the PDCD1 gene into M-07e cells using genetic engineering techniques. This typically involves cloning the PDCD1 gene into a suitable expression vector and then transfecting the vector into M-07e cells. Successfully transfected cells are then selected to form a cell line that stably expresses PDCD1.

How can the role of PDCD1 in tumor immunotherapy be studied using the Human PDCD1 Stable Cell Line - M-07e?

A: The role of PDCD1 in tumor immunotherapy can be investigated by modulating its expression levels in the Human PDCD1 Stable Cell Line - M-07e. For example, RNA interference or CRISPR/Cas9 technology can be used to knock down or knockout the PDCD1 gene, and observe changes in tumor cell growth, migration, and invasion abilities. In addition, PDCD1 can be expressed in various types of tumor cells to study its functions in tumor immunotherapy.

What are the applications of the Human PDCD1 Stable Cell Line - M-07e in drug screening?

A: The Human PDCD1 Stable Cell Line - M-07e can be used for screening potential drugs targeting PDCD1. For example, high-throughput screening methods can be employed to identify small molecule compounds that can inhibit PDCD1 activity or expression. These compounds can then be further studied for their antitumor activities, providing a foundation for the development of novel antitumor drugs.

How can the stability and activity of the Human PDCD1 Stable Cell Line - M-07e be ensured during experimental procedures?

A: To ensure the stability and activity of the Human PDCD1 Stable Cell Line - M-07e during experiments, strict aseptic techniques and appropriate culture conditions, such as temperature, humidity, and CO2 concentration, should be followed. Regular testing of the cells should be conducted to ensure they are free from contamination or genetic drift. In addition, avoid excessive passaging of the cells to maintain their stability.

What are the advantages of using the Human PDCD1 Stable Cell Line - M-07e to study the interaction between PDCD1 and other signaling pathways?

A: The advantages of using the Human PDCD1 Stable Cell Line - M-07e to study the interaction between PDCD1 and other signaling pathways include: 1) stable expression of PDCD1, which facilitates the investigation of its role in signaling pathways; 2) the ability to observe the effects of PDCD1 on signaling pathways at the cellular level, revealing its functions in biological processes; 3) the possibility to further study the interaction between PDCD1 and other members of signaling pathways using gene editing technologies in this cell line.

How to choose control when using the Human PDCD1 Stable Cell Line - M-07e for experiments?

A: When selecting control groups for experiments using the Human PDCD1 Stable Cell Line - M-07e, consider the following strategies: 1) use untransfected M-07e cells as controls to exclude the effects of other factors on the experimental results; 2) use M-07e cells transfected with an empty vector as controls to evaluate the contribution of PDCD1 expression to the results; 3) if studying the role of PDCD1 under specific conditions, other stable cell lines with similar characteristics can be used as controls.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *