Human PDCD1 Stable Cell Line-CHO-K1

Name: Human PDCD1 Stable Cell Line-CHO-K1
SKU: CSC-RO0069
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0069

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0069
Description	This cell line is a stably transfected cell line which expresses human programmed cell death 1 (PDCD1).
Background	PDCD1 protein is a cell surface membrane protein of the immunoglobulin superfamily. It is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.
Target	Human
Target Gene	PDCD1
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Source	CHO-K1
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PDCD1 programmed cell death 1 [ Homo sapiens (human) ]
Gene Symbol	PDCD1
Synonyms	PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
Gene ID	5133
Uni Prot ID	Q15116
Chromosome Location	2q37.3

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How to enhance cell proliferation of Human PDCD1 Stable Cell Line-CHO under serum-free culture conditions?

A: Gradually adapt to serum-free culture or add specific serum-free growth factors and nutritional supplements to promote cell proliferation.

How to enhance the detection signal of surface PDCD1 in flow cytometry analysis of Human PDCD1 Stable Cell Line-CHO?

A: Optimize the choice and dilution ratio of antibodies, extend the incubation time with cells, and ensure that the cells remain viable before analysis.

How to improve co-immunoprecipitation efficiency in protein interaction studies with Human PDCD1 Stable Cell Line-CHO?

A: Optimize cell lysis conditions, use efficient immunoprecipitation reagents and appropriate antibodies for PDCD1 protein, ensure sufficient incubation time, and gentle washing steps.

How to improve gene silencing experiments in Human PDCD1 Stable Cell Line-CHO?

A: Optimize the design of siRNA or shRNA to ensure specificity for the PDCD1 gene, and adjust transfection efficiency and dosage.

How to improve the consistency of drug sensitivity testing in Human PDCD1 Stable Cell Line-CHO?

A: Ensure uniform experimental conditions, including cell density, drug concentration, and duration of action, and perform multiple repetitions to increase the reliability of the data.

How to optimize cell viability in apoptosis experiments with Human PDCD1 Stable Cell Line-CHO?

A: Check and optimize culture conditions, reduce physical or chemical stress factors, and use cell protectants to minimize apoptosis.

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