Human PDCD1 Stable Cell Line - Daudi

Name: Human PDCD1 Stable Cell Line - Daudi
SKU: CSC-RO0209
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0209

Host Cell : Daudi Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0209
Description	This cell line is engineered to stably overexpress the human PDCD1 in Daudi cells.
Introduction	This cell line is constructed by transfection of human programmed cell death 1 (PDCD1) into Daudi, followed by stable cell selection. The expression of PDCD1 has been analyzed through flow cytometry.
Target Gene	PDCD1
Gene Species	Homo sapiens (Human)
Host Cell	Daudi
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PDCD1 programmed cell death 1 [ Homo sapiens ]
Gene Symbol	PDCD1
Synonyms	PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l
Gene ID	5133
Uni Prot ID	Q15116
m RNA Refseq	NM_005018.2
Protein Refseq	NP_005009.2
Chromosome Location	2q37.3
Function	protein binding; signal transducer activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Immune System, organism-specific biosystem; PD-1 signaling, organism-specific biosystem; T cell receptor signaling pathway, organism-specific biosystem;
MIM	600244

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