Cat.No. : CSC-RO0203 Host Cell: CHO-K1
| Cat. No. | CSC-RO0203 |
| Description | This cell line is engineered to stably overexpress the human FLT3 in CHO-K1 cells. |
| Introduction | This cell line is constructed by transfection of human fms related tyrosine kinase 3 (FLT3) into CHO-K1, followed by stable cell selection. The expression of FLT3 has been analyzed through flow cytometry. |
| Gene | FLT3 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human FLT3 Stable Cell Line - CHO-K1 offers a controlled and stable environment to study the FLT3 signaling pathways. The CHO-K1 cells, with their well-defined genetic background, allow researchers to focus on FLT3-specific effects without the confounding influence of other variables. This cell line can be used to investigate how FLT3 mutations lead to the dysregulation of normal hematopoiesis and contribute to the development of leukemia. Additionally, the system can be utilized to test the efficacy of FLT3 inhibitors, which are crucial for the development of targeted therapies for AML and other FLT3-associated disorders.
A: By utilizing the Human FLT3 Stable Cell Line - CHO-K1, researchers can perform in vitro cytotoxicity assays to evaluate the effects of FLT3 inhibitors on cell viability and proliferation. This cell line, with its stable FLT3 expression, provides a consistent and reliable model to assess the IC50 values (concentration of a drug that inhibits cellular growth by 50%) of potential FLT3 inhibitors. The data obtained from these assays can guide further preclinical and clinical development of these therapeutic agents, ultimately contributing to more effective treatments for patients with FLT3-mutated cancers.
A: The Human FLT3 Stable Cell Line - CHO-K1 is a valuable tool for studying the mechanisms of resistance to FLT3 inhibitors. By culturing these cells under selective pressure with FLT3 inhibitors, researchers can identify genetic changes and alternative signaling pathways that confer resistance. This knowledge is essential for the rational design of next-generation FLT3 inhibitors that can overcome resistance and provide more durable therapeutic responses in the treatment of hematological malignancies.
A: The Human FLT3 Stable Cell Line - CHO-K1 can be used to explore the complex interplay between FLT3 and other signaling pathways that contribute to cancer development and progression. By manipulating the expression or activity of components in these pathways within the CHO-K1 cells, researchers can investigate how these interactions influence FLT3 signaling and cell behavior. This can lead to a better understanding of the integrated network of signaling pathways in cancer and inform the development of combination therapies that target multiple pathways simultaneously.
A: The Human FLT3 Stable Cell Line - CHO-K1 is an ideal platform for the development of high-throughput screening assays to identify novel FLT3 modulators. By incorporating reporter genes under the control of FLT3-responsive promoters or using assays that measure FLT3 phosphorylation and downstream signaling events, researchers can screen large libraries of compounds for their ability to modulate FLT3 activity. This approach can lead to the discovery of new therapeutic targets and the development of innovative treatments for diseases driven by FLT3 dysregulation.
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With the stable integration of FLT3, this cell line is well-suited for mapping the downstream signaling pathways activated by FLT3 engagement. The Human FLT3 Stable Cell Line - CHO-K1 allows us to trace the cascade of events following FLT3 activation, providing valuable insights into the cellular and molecular mechanisms mediated by this receptor.
The consistent expression of FLT3 makes the Human FLT3 Stable Cell Line - CHO-K1 an excellent tool for biomarker studies. We can use this cell line to identify and validate biomarkers related to FLT3 expression and activity, which are critical for diagnosing and monitoring FLT3-positive leukemias.
The Human FLT3 Stable Cell Line - CHO-K1 enables targeted studies on FLT3 signaling pathways, crucial for understanding its role in the development and progression of blood cancers like acute myeloid leukemia (AML). This cell line allows us to dissect FLT3's contributions to cellular processes, enhancing the understanding of hematological malignancies.
This cell line is specifically designed for testing drugs that target FLT3, a receptor tyrosine kinase implicated in the proliferation and survival of leukemia cells. The Human FLT3 Stable Cell Line - CHO-K1 provides a consistent and reliable model for evaluating the efficacy and specificity of FLT3 inhibitors.
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