Cat.No. : CSC-RO0068 Host Cell: CHO-K1
| Cat. No. | CSC-RO0068 |
| Description | This cell line is a stably transfected cell line which expresses mouse FMS-like tyrosine kinase 3 (Flt3) without any tag. |
| Background | Mice functionally null for this gene display abnormal lymphopoiesis. Homozygous ENU mutant mice are sensitive to infection by mouse cytomegalovirus. |
| Gene | Flt3 |
| Gene Species | Mus musculus (Mouse) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Target | Mouse |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | Flt3 FMS-like tyrosine kinase 3 [ Mus musculus (house mouse) ] |
| Gene Symbol | Flt3 |
| Synonyms | Flk2; Ly72; wmfl; CD135; Flk-2; Flt-3; B230315G04 |
| Gene ID | 14255 |
| UniProt ID | Q00342 |
| Chromosome Location | 5 86.88 cM; 5 G3 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Mouse FLT3 Stable Cell Line-CHO is derived from engineering and stably expressing the FLT3 gene from a mouse source in CHO (Chinese Hamster Ovary) cells. This cell line is typically created by cloning the FLT3 gene from mouse DNA and then inserting it into specific loci in CHO cells through selective culturing and screening. The process involves a combination of genetic engineering techniques and cell culture methods.
A: The genotype of Mouse FLT3 Stable Cell Line-CHO includes the CHO cell's own genome and the stably expressed mouse FLT3 gene. In terms of phenotype, these cells will express the FLT3 receptor protein, which is a cell membrane receptor typically involved in cell signaling and proliferation processes.
A: Mouse FLT3 Stable Cell Line-CHO has a wide range of applications in biomedical research and drug development. It can be used to investigate the regulation of the FLT3 signaling pathway, the functionality and activity of mouse FLT3 receptors, drug screening, and the development of anti-cancer drugs. This cell line can also be employed to study the mechanisms of leukemia and other related diseases, as well as to assess the effectiveness of new drugs.
A: Yes, the use of Mouse FLT3 Stable Cell Line-CHO is subject to regulatory and ethical requirements. When conducting research and drug development, it is necessary to adhere to relevant regulations, ethical standards, and laboratory safety guidelines to ensure the lawful use of the cell line and the ethics of experiments.
A: Mouse FLT3 Stable Cell Line-CHO is typically stored in liquid nitrogen to ensure long-term stability. To revive these cells, they are usually rapidly thawed at 37 degrees Celsius and seeded into culture dishes using appropriate culture media and conditions.
A: Biosafety precautions for Mouse FLT3 Stable Cell Line-CHO should be taken based on the usage environment and experimental requirements. This includes following biosafety level (BSL) standards in the laboratory, using appropriate protective equipment, taking appropriate measures when handling biohazardous materials, and regularly cleaning and disinfecting cell culture vessels and equipment.
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