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Mouse FLT3 Stable Cell Line-CHO-K1

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RO0068

Host Cell :   CHO-K1 Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RO0068
Description This cell line is a stably transfected cell line which expresses mouse FMS-like tyrosine kinase 3 (Flt3) without any tag.
Background Mice functionally null for this gene display abnormal lymphopoiesis. Homozygous ENU mutant mice are sensitive to infection by mouse cytomegalovirus.
Target Mouse
Target Gene Flt3
Gene Species Mus musculus (Mouse)
Host Cell CHO-K1
Host Cell Species Cricetulus griseus (Chinese hamster)
Source CHO-K1
Applications

1. Studying the interactions between immune cells and cancer cells

2. Studying the mechanisms of resistance to immune checkpoint blockade

3. High-throughput screening

4. Drug target validation

Size >1x106 frozen cells/vial
Stability Validated for at least 10 passages
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name Flt3 FMS-like tyrosine kinase 3 [ Mus musculus (house mouse) ]
Gene Symbol Flt3
Synonyms Flk2; Ly72; wmfl; CD135; Flk-2; Flt-3; B230315G04
GeneID 14255
Uni ProtID Q00342
Chromosome Location 5 86.88 cM; 5 G3
Quick Inquiry

Q & A

Customer Reviews

Customer Q&As
What is the origin of Mouse FLT3 Stable Cell Line-CHO, and how was it established?

A: Mouse FLT3 Stable Cell Line-CHO is derived from engineering and stably expressing the FLT3 gene from a mouse source in CHO (Chinese Hamster Ovary) cells. This cell line is typically created by cloning the FLT3 gene from mouse DNA and then inserting it into specific loci in CHO cells through selective culturing and screening. The process involves a combination of genetic engineering techniques and cell culture methods.

What is the genotype and phenotype of Mouse FLT3 Stable Cell Line-CHO?

A: The genotype of Mouse FLT3 Stable Cell Line-CHO includes the CHO cell's own genome and the stably expressed mouse FLT3 gene. In terms of phenotype, these cells will express the FLT3 receptor protein, which is a cell membrane receptor typically involved in cell signaling and proliferation processes.

What are the applications of Mouse FLT3 Stable Cell Line-CHO in scientific research and drug development?

A: Mouse FLT3 Stable Cell Line-CHO has a wide range of applications in biomedical research and drug development. It can be used to investigate the regulation of the FLT3 signaling pathway, the functionality and activity of mouse FLT3 receptors, drug screening, and the development of anti-cancer drugs. This cell line can also be employed to study the mechanisms of leukemia and other related diseases, as well as to assess the effectiveness of new drugs.

Is Mouse FLT3 Stable Cell Line-CHO subject to regulatory and ethical requirements?

A: Yes, the use of Mouse FLT3 Stable Cell Line-CHO is subject to regulatory and ethical requirements. When conducting research and drug development, it is necessary to adhere to relevant regulations, ethical standards, and laboratory safety guidelines to ensure the lawful use of the cell line and the ethics of experiments.

What are the methods for storage and revival of Mouse FLT3 Stable Cell Line-CHO?

A: Mouse FLT3 Stable Cell Line-CHO is typically stored in liquid nitrogen to ensure long-term stability. To revive these cells, they are usually rapidly thawed at 37 degrees Celsius and seeded into culture dishes using appropriate culture media and conditions.

What are the specific biosafety considerations for Mouse FLT3 Stable Cell Line-CHO?

A: Biosafety precautions for Mouse FLT3 Stable Cell Line-CHO should be taken based on the usage environment and experimental requirements. This includes following biosafety level (BSL) standards in the laboratory, using appropriate protective equipment, taking appropriate measures when handling biohazardous materials, and regularly cleaning and disinfecting cell culture vessels and equipment.

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Customer Reviews
Excellent Tool for FLT3-Targeted Screening

The Mouse FLT3 Stable Cell Line in CHO-K1 cells has been a game-changer for our drug screening projects. The receptor expression level is stable over many generations, and the cells respond robustly to known FLT3 ligands. It saved us significant time compared to transient transfection systems. Highly recommended for anyone studying FLT3 signaling.

Italy

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