Cat.No. : CSC-RO0204 Host Cell: CHO-K1
| Cat. No. | CSC-RO0204 |
| Description | This cell line is engineered to stably overexpress the monkey FLT3 in CHO-K1 cells. |
| Introduction | This cell line is constructed by transfection of monkey fms related tyrosine kinase 3 (FLT3) into CHO-K1, followed by stable cell selection. The expression of FLT3 has been analyzed through flow cytometry. |
| Gene | FLT3 |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: This cell line mirrors the FLT3 receptor's expression and signaling in a primate context, allowing for cross-species comparisons of FLT3-mediated cellular processes and responses to FLT3 inhibitors.
A: By treating the cells with recombinant FLT3 ligand and measuring proliferation rates using assays like MTT or cell counting, the proliferative response to ligand stimulation can be quantified.
A: Yes, by introducing mutations known to cause resistance and treating cells with various inhibitors, one can assess the efficacy of the drugs against the mutated receptors.
A: Regular testing using flow cytometry or Western blot to confirm the expression of FLT3 at the cell surface or protein level, respectively, is recommended.
A: It provides a relevant biological system to test the efficacy of novel FLT3 inhibitors or to study the mechanism of action and resistance, which is critical in the development of targeted leukemia therapies.
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This stable cell line ensures consistent expression of the FLT3 receptor, which is crucial for obtaining reliable and reproducible results in experiments.
The CHO-K1 cells provide a high level of clonal purity, which is essential for minimizing variability and ensuring the stability of the FLT3 receptor expression.
The Monkey FLT3 Stable Cell Line - CHO-K1 is relatively easy to culture and maintain, making it accessible for labs with varying levels of expertise in cell culture techniques.
The stable expression of FLT3 in CHO-K1 cells allows for more precise research into FLT3-dependent processes, which can be particularly useful for drug discovery efforts.
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