Human FLT3 Stable Cell Line-Ba/F3

Name: Human FLT3 Stable Cell Line-Ba/F3
SKU: CSC-RO0237
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0237

Host Cell : Ba/F3 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0237
Description	This cell line is engineered to stably express human FLT3 gene.
Target Gene	FLT3
Gene Species	Homo sapiens (Human)
Host Cell	Ba/F3
Host Cell Species	Mus musculus (Mouse)
Applications	Drug screening and biological assays
Biosafety Level	2
Size	>1x10^6 frozen cells/vial, 1 mL
Stability	Stable in culture over a minimum of 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Growth Conditions	37 °C, 5% CO₂
Storage	Liquid nitrogen
Shipping	Dry ice

Thawing & Subculturing Instructions	1. Thaw cells by gently swirling in a 37°C water bath. To limit contamination, do not submerge the O-ring and cap. 2. When cells are ~70% thawed (~1 min), transfer the vial into a biosafety cabinet, and wipe the surface with 70% ethanol. Allow tube to dry completely. 3. Transfer the cells gently into a 15 mL conical tube containing 10 mL of pre-warmed culture medium (without antibiotic selection marker). Centrifuge cells at ~125 x g for 5~7 min. 4. Remove supernatant without disturbing the pellet, and resuspend cells in 1 mL culture medium (without antibiotic selection marker). Transfer cells to a 6-well plate containing ~2 mL pre-warmed growth medium (without antibiotic selection marker) or a T25 flask containing 5 mL pre-warmed culture medium (without antibiotic selection marker). 5. Incubate the culture at 37°C with 5% CO2. 6. Subculture: split saturated culture 1:4 ~ 1:6 every 3 days; seed out at about 1~3 x 10^5 cells/mL.
Freeze Medium	Frozen with 70% medium, 20% FBS, 10% DMSO
Growth Properties	Suspension, round
Freezing Instructions	Cells are recommended to generate additional frozen stocks at early passages. Frozen stocks should be preserved in a designated cryopreservation medium or in 70% RPMI 1640 + 20% FBS + 10% DMSO (without antibiotic selection marker). 1. Prepare the freezing medium (70% RPMI 1640 + 20% FBS + 10% DMSO, without antibiotic selection marker) fresh immediately before use. 2. Keep the freezing medium on ice and label cryovials. 3. Transfer cells to a sterile, conical centrifuge tube, and count the cells. 4. Centrifuge the cells at 250 x g for 5 minutes at room temperature and carefully aspirate off the medium. 5. Resuspend the cells at a density of at least 3 x10^6 cells/ml in chilled freezing medium. 6. Aliquot 1 ml of the cell suspension into each cryovial. 7. Freeze cells in the CoolCell freezing container overnight in a -80°C freezer. 8. Transfer vials to liquid nitrogen for long-term storage.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	FLT3 fms-related tyrosine kinase 3 [ Homo sapiens ]
Gene Symbol	FLT3
Synonyms	FLK2; STK1; CD135; FLK-2
Gene Description	fms-related tyrosine kinase 3
GeneID	2322
Uni ProtID	P36888
mRNA Refseq	NM_004119.2
Protein Refseq	NP_004110.2
Chromosome Location	13q12
Function	ATP binding; cytokine receptor activity; phosphatidylinositol 3-kinase binding; protein binding; protein homodimerization activity; transmembrane receptor protein tyrosine kinase activity; vascular endothelial growth factor-activated receptor activity;
Pathway	Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Pathways in cancer, organism-specific biosystem;
MIM	136351

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Background

Applications

Q & A

Customer Reviews

FLT3 (or FMS-related tyrosine kinase 3) is a protein that is critical for the maturation of hematopoietic progenitor cells (cells that eventually develop into blood cells). FLT3 plays a crucial role in cell development, particularly in the production and development of blood cells in the bone marrow. In a healthy body, the FLT3 gene helps control how quickly and how many cells the body produces. However, mutations in the FLT3 gene can lead to the overproduction of immature blood cells, a condition that can lead to acute myeloid leukemia (AML). Due to its association with cancer, FLT3 has become an important area of research in cancer research. There are currently several therapies and drugs that target FLT3 in development with the goal of treating or preventing leukemia and other similar diseases.

Human FLT3 Stable Cell Line-Ba/F3 is engineered to stably integrate the human FLT3 gene into Ba/F3 cells. This specific feature of the human FLT3 stable cell line-Ba/F3 is particularly useful for the study of AML, as well as drug screening studies to identify potential therapeutic targets in this process. This cell line provides researchers with an important tool to understand the function of FLT3 and the effects of mutations.

The human FLT3 stable cell line-Ba/F3 is widely used in biomedical research, more specifically in the field of cancer research. Here are a few key points of its application:

Drug screening: This cell line is commonly used to screen new drugs and small molecules targeting FLT3. This cell line expresses large amounts of FLT3 protein, making it an ideal model for studying the efficacy of drugs against FLT3 mutant cells.

Molecular biology studies: This cell line is used to study the molecular biology of the FLT3 protein, specifically its mutation status.

Cancer research: FLT3 mutations associated with acute myeloid leukemia (AML). The Ba/F3 cell line expressing human FLT3 was used to study the role of this protein in the pathogenesis of AML, which could lead to the development of new therapeutic strategies for this disease.

Genetic research: The human FLT3 stable cell line is used as a tool for genetic research, particularly in understanding the relationship between certain genes and different forms of leukemia.

Protein interaction studies: These cells can be used for protein-protein interaction studies to gain insight into the FLT3 signaling pathway.

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Customer Reviews

Robust Expression of FLT3

Robust and stable expression of FLT3 in these cells is critical for our targeted drug development programs. We have successfully used this cell line to identify several promising FLT3 inhibitors, which has greatly accelerated our research progress.

Germany

2020-05-13

High Survival

This cell line exhibits excellent survival and proliferation rates, which are critical for our high-throughput screening assays. This allows us to perform multiple parallel tests without the common problem of cell line degradation affecting our results.

Germany

2021-11-12

Excellent consistency

We have been using the Human FLT3 Stable Cell Line-Ba/F3 for several months in our oncology research lab and the consistency of the results has been remarkable. Each batch performs exactly the same as the previous one, which greatly increases the reliability of our experiments.

Canada

2023-07-16

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Human FLT3 Stable Cell Line-Ba/F3

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Applications

Q & A

Customer Reviews

Robust Expression of FLT3

High Survival

Excellent consistency

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