Human FLT3 Stable Cell Line - HEK293T

Name: Human FLT3 Stable Cell Line - HEK293T
SKU: CSC-RO0963
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0963

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0963
Description	This cell line is engineered to stably overexpress Human FLT3 in HEK293T cells.
Target Gene	FLT3
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	Drug screening and biological assays
Size	>1x10^6 frozen cells/vial, 1 mL
Stability	Stable in culture over a minimum of 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	FLT3 fms-related tyrosine kinase 3 [ Homo sapiens ]
Gene Symbol	FLT3
Synonyms	FLK2; STK1; CD135; FLK-2
Gene Description	fms-related tyrosine kinase 3
GeneID	2322
Uni ProtID	P36888
mRNA Refseq	NM_004119.2
Protein Refseq	NP_004110.2
Chromosome Location	13q12
Function	ATP binding; cytokine receptor activity; phosphatidylinositol 3-kinase binding; protein binding; protein homodimerization activity; transmembrane receptor protein tyrosine kinase activity; vascular endothelial growth factor-activated receptor activity;
Pathway	Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Pathways in cancer, organism-specific biosystem;
MIM	136351

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