Easy-Change® XL Site-Directed Mutagenesis Kit

Name: Easy-Change® XL Site-Directed Mutagenesis Kit
SKU: SMK-Y001
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : SMK-Y001

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Product Information

Cat. No.	SMK-Y001
Description	Creative Biogene's Easy-Change® XL Site-Directed Mutagenesis Kit allows site specific mutation in virtually any double-stranded plasmid, thus eliminating the need for subcloning into M13-based bacteriophage vectors and for ssDNA rescue. In addition, the Easy-Change® XL system requires no specialized vectors, unique restriction sites, or multiple transformations. This rapid four-step procedure generates mutants with greater than 80% efficiency. The protocol is simple and uses either miniprep plasmid DNA or cesium-chloride-purified DNA.
Sample Type	DNA
Features	• Designed for more efficient DNA replication and bacterial transformation. • Allows sitespecific mutation in virtually any double-stranded plasmid. • Requires no specialized vectors, unique restriction sites, or multiple transformations. • Rapid four-step procedure generates mutants with greater than 80% efficiency.
Applications	• Study of protein structure-function relationships and gene expression; • Vector modification;
Storage	Store at -20°C. When stored as directed, the product is stable for at least two years.

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What is site-directed mutagenesis?

A: Site-directed mutagenesis is a laboratory technique used to introduce specific mutations or changes in a DNA sequence at a desired site. It involves altering the genetic code of a gene by introducing specific nucleotide changes to study the effects of these mutations on protein structure and function.

What is the efficiency of site-directed mutagenesis kit?

A: A mutagenesis efficiency >80% was consistently achieved in the case of two unrelated plasmids.

What are the benefits of site-directed mutagenesis?

A: Site-directed mutagenesis is a valuable tool for modifying genes and studying the structural and functional properties of proteins based on the structure, function, catalytic mechanism, and catalytic residues of enzymes. Site-directed mutations include single mutations and combination mutations.

Why are the desired mutations missing from my screened transformants?

A: Although this is not a common result, there are several known causes of background transformants: 1) too many plasmids in the PCR, 2) selection against specific DNA structures (such as inverted or tandem repeats), and 3) selection against the recombinant protein by the host E. coli cells.

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Customer Reviews

Worked very well

Extremely low error rate reduces screening time. Good experimental results were obtained.

United States

2022-01-27

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Easy-Change® XL Site-Directed Mutagenesis Kit

Product Information

Q & A

Customer Reviews

What is site-directed mutagenesis?

What is the efficiency of site-directed mutagenesis kit?

What are the benefits of site-directed mutagenesis?

Why are the desired mutations missing from my screened transformants?

Worked very well

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