EZ Random Mutagenesis Kit

Name: EZ Random Mutagenesis Kit
SKU: RMK-L001
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : RMK-L001

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Product Information

Cat. No.	RMK-L001
Description	Creative Biogene's EZ Random Mutagenesis Kit is designed for performing PCR-based random mutagenesis on a DNA sequence of interest. This kit contains an optimized Taq PCR system which is able to reliably amplify DNA fragments of up to 4 kb in length and provide mutation rates from 2 to 8 mutations per kb. This kit is ideal for studying, altering, or improving wild-type protein function.
Sample Type	DNA
Features	• Controlled random mutagenesis: you can select the desired rate of mutation by choosing from a set of predefined experimental conditions. • Amplification of large PCR fragments: the kit has been confirmed amplification of fragments up to 4 kb in length. Theoretically, longer fragments can be mutated and amplified as well. • High DNA yields.
Applications	• Random mutagenesis; • Study of protein structure-function relationships; • improve or alter the characteristics of wild-type proteins;
Storage	Store at -20°C. When stored as directed, the product is stable for one year.

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What is the difference between random and directed mutagenesis?

A: In random mutagenesis, the mutation occurring on the DNA site is random. Any random site can be chosen for mutation. In site-directed mutagenesis, the site of mutation is on the specific target sites.

Why is random mutagenesis important?

A: Mutagenesis is an important technique in protein engineering to alter properties such as thermal stability, pH optimum, and specific activity, or to understand the structure-function relationships of a target protein.

How to achieve random mutagenesis?

A: This can be achieved by treating DNA or whole bacteria with various chemical mutagens, by passing cloned genes through mutator strains, by "error-prone" PCR mutagenesis, by rolling circle error-prone PCR, or by saturation mutagenesis.

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Customer Reviews

Great!

The EZ Random Mutagenesis kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis.

United States

2020-08-23

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EZ Random Mutagenesis Kit

Product Information

Q & A

Customer Reviews

What is the difference between random and directed mutagenesis?

Why is random mutagenesis important?

How to achieve random mutagenesis?

Great!

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