Easy Cloning Unbiased Random Mutagenesis Kit

RMK-L003

Creative Biogene's Easy Cloning Unbiased Random Mutagenesis Kit provides an easy and fast cloning method to perform targeted random mutagenesis on protein domains and promoter elements. This kit contains a novel error-prone PCR enzyme which is able to produce a less biased mutational spectrum with equivalent mutation rates at A's and T's vs. G's and C's and provide mutation rates from 1 to 16 mutations per kb. This kit allows you to target specific protein domains or promoter elements without the need for restriction sites or sub-cloning.

• Random mutagenesis;
• Study of protein structure-function relationships;
• improve or alter the characteristics of wild-type proteins;

• Unbiased random mutagenesis: The kit is able to produce a less biased mutational spectrum with equivalent mutation rates at A's and T's vs. G's and C's.
• Controlled random mutagenesis: you can select the desired rate of mutation by choosing from a set of experimental conditions.
• Amplification of large PCR fragments: the kit can be used to mutagenize plasmid DNA targets up to 3.5 kb in length.

DNA

Store the competent cells immediately at -80°C. All other components store at -20°C upon receipt.