Random Mutagenesis Kit

Name: Random Mutagenesis Kit
SKU: RMK-L004
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : RMK-L004

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Product Information

Cat. No.	RMK-L004
Description	Creative Biogene's Random Mutagenesis Kit is designed for performing error-prone PCR-based random mutagenesis on a DNA sequence of interest. This kit contains an optimized Taq PCR system which is available to amplify DNA fragments of less than 4 kb with GC content below 70% and provide mutation rates from 1 to 18 mutations per kb. This kit is ideal for studying, altering, or improving wild-type protein function.
Sample Type	DNA
Features	• Easy to operate: the kit contains an optimized premix system that can reduce errors and contamination opportunities due to multiple sample additions. • Controlled random mutagenesis: you can select the desired rate of mutation by choosing from a set of experimental conditions. • Amplification of large PCR fragments.
Applications	• Random mutagenesis; • Study of protein structure-function relationships; • improve or alter the characteristics of wild-type proteins;
Storage	Store at -20°C. When stored as directed, the product is stable for one year.

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What is random mutation?

A: Random mutation refers to the spontaneous, random changes in the genetic material (DNA) of an organism. These mutations can occur naturally during DNA replication or as a result of external factors such as radiation or chemical exposure.

What is error-prone PCR?

A: Error-prone PCR (Polymerase Chain Reaction) is a laboratory technique used to introduce random mutations or errors into DNA sequences during the amplification process.

What is the principle of error-prone PCR to achieve random mutagenesis?

A: Error-prone PCR is typically performed using a modified DNA polymerase that lacks proofreading abilities. This polymerase has an increased error rate in copying the template DNA compared to regular DNA polymerases.

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Customer Reviews

Superior yields

Great results. Superior yields over a broad target range - 0.1 to 4 kb.

Germany

2021-10-09

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Random Mutagenesis Kit

Product Information

Q & A

Customer Reviews

What is random mutation?

What is error-prone PCR?

What is the principle of error-prone PCR to achieve random mutagenesis?

Superior yields

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