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Unbiased Random Mutagenesis Kit

For research use only. Not intended for any clinical use.
Cat.No.
RMK-L002
Description
Creative Biogene's Unbiased Random Mutagenesis Kit is designed for performing error-prone PCR-based random mutagenesis on a DNA sequence of interest. This kit contains a novel error-prone PCR enzyme which is able to produce a less biased mutational spectrum with equivalent mutation rates at A's and T's vs. G's and C's and provide mutation rates from 1 to 16 mutations per kb. This kit is ideal for studying, altering, or improving wild-type protein function.
Applications
• Random mutagenesis;
• Study of protein structure-function relationships;
• improve or alter the characteristics of wild-type proteins;
Features
• Unbiased random mutagenesis: The kit is able to produce a less biased mutational spectrum with equivalent mutation rates at A's and T's vs. G's and C's.
• Controlled random mutagenesis: you can select the desired rate of mutation by choosing from a set of experimental conditions.
• Amplification of large PCR fragments: the kit can be used to mutagenize plasmid DNA targets up to 6 kb in length.
Sample Type
DNA
Storage
Store at -20°C.

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Q & A

Customer Reviews

Customer Q&As
What is unbiased random mutagenesis?

A: Unbiased random mutagenesis refers to a technique used in molecular biology to introduce random mutations into a DNA sequence with the aim of studying gene function or protein structure and function. This method involves creating genetic diversity by introducing random mutations throughout the DNA sequence, without having any preference for specific locations or types of mutations.

What are the types of PCR-based mutagenesis?

A: The main strategies of PCR-based mutagenesis include base substitution, deletion, insertion, chimeric gene generation, multiple-site mutagenesis, and random mutagenesis at either a single site or multiple sites.

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Customer Reviews
High efficiency

Easily change mutation frequency by altering concentration of input template. I like it.

United States

05/12/2021

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