Panoply™ Human MSLN Over-expressing Stable Cell Line

Name: Panoply™ Human MSLN Over-expressing Stable Cell Line
SKU: CSC-SC009829
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC009829

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC009829
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	MSLN
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	MSLN mesothelin [ Homo sapiens ]
Gene Symbol	MSLN
Synonyms	MSLN; mesothelin; CAK1; MPF; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor; SMRP;
Gene ID	10232
Uni Prot ID	Q13421
m RNA Refseq	BC009272
Chromosome Location	16p13.3
MIM	601051

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Lung cancer and pleural mesothelioma are two of the most lethal cancers. Limited treatment options and a poor understanding of disease mechanisms contribute to the extremely poor prognosis of lung cancer and mesothelioma. Mesothelin (MSLN) is a plasma membrane differentiation antigen expressed at high levels in many human solid tumors, including 70% of lung cancers and nearly all mesotheliomas. Here, researchers demonstrate that MSLN plays a critical role in controlling the epithelial-mesenchymal transition (EMT) and stemness properties of human lung cancer and mesothelioma cells, which dictate their tumorigenic and metastatic potential. First, they found that MSLN is highly upregulated in non-small cell lung cancer (NSCLC) patient tissues and in lung cancer and mesothelioma cell lines. Second, genetic knockdown of MSLN significantly reduced anchorage-independent cell growth, tumorsphere formation, cell adhesion, migration, and invasion in vitro, as well as tumor formation and metastasis in vivo. Third, ectopic overexpression of MSLN induced a malignant phenotype in non-cancerous cells, supporting its role as an oncogene. Finally, mechanistic studies revealed that knockdown of MSLN reversed EMT and attenuated stem cell properties, in addition to inhibiting tumor growth and metastasis. These results suggest that MSLN plays an important role in controlling EMT and stem cell properties in human lung cancer and mesothelioma cells.

Here, researchers performed gene overexpression experiments to compare the effects of MSLN overexpression on the anchorage-independent growth, migration, and invasion of noncancerous MeT5A mesothelial cells. The results showed that control MeT5A cells formed few or no small colonies in soft agar, whereas MSLN-overexpressing cells (MeT5A/MSLN) formed multiple large colonies (Figures 1a-c). Cell migration studies also demonstrated that MSLN-overexpressing cells were more migratory and invasive than control cells (Figures 1d-g). Together, these findings support a tumorigenic/metastatic role for MSLN in the tested cell systems.

Figure 1. Overexpression of MSLN promotes colony formation, cell migration, and invasion. (He X, et al., 2017)

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