Human MSLN Stable Cell Line - U87

Name: Human MSLN Stable Cell Line - U87
SKU: CSC-RO0265
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0265

Host Cell : U87 Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0265
Description	Human MSLN Stable Cell Line - U87 is engineered to stably overexpress human mesothelin (MSLN).
Target Gene	MSLN
Gene Species	Homo sapiens (Human)
Host Cell	U87
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Homo sapiens mesothelin DNA.
Gene Symbol	MSLN
Synonyms	MPF; SMRP; MSLN

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What is the origin of Human MSLN Stable Cell Line - U87, and how was it established?

A: Human MSLN Stable Cell Line - U87 is derived from the human glioblastoma cell line U87. U87 cells were initially isolated and established from human brain tissue in 1968. To establish the Human MSLN Stable Cell Line - U87, it is likely that U87 cells were transfected or stably engineered to express the mesothelin (MSLN) gene.

What are the genetic and phenotypic characteristics of Human MSLN Stable Cell Line - U87?

A: The genetic and phenotypic characteristics of Human MSLN Stable Cell Line - U87 may vary depending on the specific manipulations in the laboratory, but generally, they retain the genetic and phenotypic characteristics of U87 cells. U87 cells belong to the glioblastoma cell line and typically exhibit features of glial cells, such as a stellate glial cell morphology and the expression of neuronal markers.

What are the primary applications of Human MSLN Stable Cell Line - U87 in research?

A: Human MSLN Stable Cell Line - U87 has various research applications, including: Tumor biology research: It can be used to investigate the role of mesothelin (MSLN) in tumor development and metastasis, as well as therapeutic strategies targeting MSLN. Drug screening: It can be employed for screening anti-tumor drugs or drugs targeting MSLN. Immunotherapy research: It is used to assess the efficacy of immunotherapeutic strategies such as immune checkpoint inhibitors against tumor cells.

What is the biosafety level of Human MSLN Stable Cell Line - U87?

A: The biosafety level of Human MSLN Stable Cell Line - U87 is typically BSL-2, which stands for Biosafety Level 2. This means that appropriate biosafety measures should be taken when handling these cells to prevent potential infections, but extremely high-level biosafety precautions are usually not required.

What are the specific research considerations to be aware of when using Human MSLN Stable Cell Line - U87?

A: When using Human MSLN Stable Cell Line - U87 for research, it is important to consider the following: Cell culture conditions: Maintain appropriate culture conditions, including temperature, CO2 concentration, and culture medium. Infection control: Implement biosafety measures to prevent cell contamination and cross-contamination.

What is the association between Human MSLN Stable Cell Line - U87 and mesothelin (MSLN), and why is this association important in research?

A: Human MSLN Stable Cell Line - U87 is associated with mesothelin (MSLN) because it is a cell line modified to express MSLN. MSLN is a membrane glycoprotein that is overexpressed in various cancer types, including pancreatic cancer, ovarian cancer, and mesothelioma. The association is important in research because it allows scientists to investigate the role of MSLN in cancer biology, explore potential therapeutic targets, and develop treatments for cancers that overexpress MSLN.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *