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Cat.No. : CSC-RO0485 Host Cell: CHO-K1
| Cat. No. | CSC-RO0485 |
| Description | This cell line is derived from CHO-K1 and is engineered to stably overexpress Human MSLN. |
| Gene | MSLN |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Lung cancer is a prevalent disease that kills one million people worldwide each year. The MSLN Stable Cell Line - CHO-K1 was utilized by researchers to investigate CAR-T treatment in non-small cell lung cancer (NSCLC) and mesothelioma. These second-generation CAR-T cells, which target mesothelin (MSLN), included co-stimulatory CD28 and 4-1BB signaling domains to increase proliferation. Flow cytometry validated the successful production of CAR-T cells, and cytotoxicity tests using the LDH kit proved the ability to kill target cells. In vivo investigations on a mouse model inoculated with tumor-derived cells from patients revealed that CAR-T MSLN cells dramatically inhibited tumor growth when injected into the tail vein, underscoring the potential and problems of CAR-T therapy in solid tumors.
Figure 1. The researchers utilized the MSLN Stable Cell Line - CHO-K1 to study MSLN expression, as evidenced by flow cytometry and LDH cytotoxicity assays. (Ye L, et al., 2019)
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Compared to traditional transient transfection or virus transduction assay kits, this product exhibits higher gene expression stability in cell lines after use. The experimental data shows that there is no significant fluctuation in the expression level of the MSLN gene, and the experimental results are reliable.
United Kingdom
08/11/2020
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