Cat.No. : CSC-RO0486 Host Cell: CHO-K1
| Cat. No. | CSC-RO0486 |
| Description | This cell line is derived from CHO-K1 and is engineered to stably overexpress Cyno MSLN. |
| Gene | MSLN |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Mesothelin is a promising target in cancer therapy, particularly in pancreatic cancer, where its overexpression is linked to tumor progression and poor prognosis. The researchers in this study focused on understanding mesothelin's role by establishing cell lines that overexpress this protein, specifically CHO-K1-MSLN and PANC-1-MSLN. These cell lines were confirmed for mesothelin expression using Western blotting, providing a robust platform for testing novel therapeutics. In parallel, the development of a fully human anti-mesothelin antibody showcased innovative therapeutic potential. The researchers used phage display technology to screen from a naïve antibody library, identifying antibodies with high specificity for mesothelin. They evaluated the binding properties of these antibodies on the established cell lines, revealing that P1A6E and P3F2 exhibited significantly higher affinity compared to traditional antibodies like SS1 and C10. Flow cytometric analysis confirmed that these scFv proteins bound effectively to mesothelin-expressing cells, underscoring their potential utility in targeted therapies.
Figure 1. The researchers established MSLN-overexpressing cell lines, CHO-K1-MSLN and PANC-1-MSLN, to evaluate the binding properties of a fully human anti-mesothelin antibody. (Jiang H, et al., 2017)
Creative Biogene's Cas9 Stable Cell Line-CFPAC-1 offers researchers a valuable tool for studying gene editing and therapeutic applications involving mesothelin and other targets. By facilitating precise modifications, this cell line supports investigations into the molecular mechanisms of cancer and the development of innovative treatments. The ability to create stable, gene-edited cell lines enhances the accuracy and reliability of experimental outcomes, driving forward the research needed to tackle challenging cancers effectively.
A: The MSLN gene encodes for the mesothelin protein, which is a glycosylated cell surface protein primarily expressed by mesothelial cells. Mesothelin functions as a cell adhesion molecule and is involved in the regulation of cell proliferation and differentiation. It is also known to be overexpressed in certain cancers, such as mesothelioma and pancreatic cancer, making it a potential target for diagnostic and therapeutic strategies.
A: The MSLN gene contributes to cancer development, particularly in mesothelioma and pancreatic cancer, by overexpression of the mesothelin protein. This overexpression can lead to aberrant cell adhesion, migration, and invasion, which are key processes in tumor progression and metastasis. Additionally, mesothelin can act as a tumor marker and facilitate the development of cancer-associated fibrosis.
A: Targeting the MSLN gene and its protein product has therapeutic implications for the treatment of cancers that overexpress mesothelin. Monoclonal antibodies, such as those directed against mesothelin, can be used to target and eliminate cancer cells. Additionally, mesothelin-targeted therapies may improve diagnostic methods and enhance the efficacy of immunotherapies.
A: The expression of the MSLN gene is regulated by various factors, including genetic alterations, oncogenic signaling, and environmental cues. In normal cells, mesothelin expression is confined to mesothelial cells. However, in cancerous cells, MSLN can become overexpressed due to genetic amplification or epigenetic changes, leading to increased mesothelin production and contributing to the pathogenesis of certain cancers.
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The Monkey MSLN Stable Cell Line - CHO-K1 complies with relevant laws and regulations regarding the use and distribution of animal cell lines. It also respects intellectual property rights, ensuring that researchers can use the cell line without infringing on any patents or copyrights.
The use of animal cell lines like the Monkey MSLN Stable Cell Line - CHO-K1 can contribute to reducing the environmental impact associated with animal experimentation. By providing an alternative in vitro model, it helps minimize the use of laboratory animals, thereby reducing ethical concerns and the environmental footprint.
The Monkey MSLN Stable Cell Line - CHO-K1 is generally well-accepted in the scientific community due to its relevance in research areas such as oncology and drug discovery. Its use aligns with the growing preference for more ethical and sustainable research practices, which is increasingly valued by society.
The Monkey MSLN Stable Cell Line - CHO-K1 is supported by comprehensive data and validation, including characterization of the cell line's phenotype, functional assays, and genetic stability. This ensures that researchers can trust the reliability and consistency of the cell line for their experiments.
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