Cat.No. : AAV00327Z
Titer: ≥1x10^12 GC/mL / ≥1x10^13 GC/mL Size: 30 ul/100 ul/500 ul/1 ml
Serotype: AAV serotype Retrograde Storage: -80 ℃
| Cat. No. | AAV00327Z |
| Description | Prepackaged AAV particles in serotype retrograde containing Cre recombinase gene under the control of human Synapsin promoter. |
| Serotype | AAV serotype Retrograde |
| Target Gene | Cre |
| Titer | Varies lot by lot, typically ≥1x10^12 GC/mL |
| Size | Varies lot by lot, for example, 30 μL, 50 μL, 100 μL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance. |
| Purity | AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE. |
| Sterility | The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities. |
| Empty vs. Full Capsids | Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods. |
Threat and extinction memories are essential for organisms to survive in changing environments. These memories are thought to be encoded by distinct neuronal populations in the brain, but their whereabouts remain elusive. Using an auditory fear conditioning and extinction paradigm in male mice, researchers identified two distinct subpopulations of projection neurons in physical proximity within the insular cortex (IC) that target the central amygdala (CeA) and nucleus accumbens (NAc), respectively, to encode fear and extinction memories. Reciprocal intracortical inhibition of these two IC subpopulations governs the emergence of fear or extinction memories. Using rabies virus-assisted tracing, researchers found that IC-NAc projection neurons are preferentially innervated by intercortical inputs from the orbitofrontal cortex (OFC), specifically enhancing extinction to override fear memories. These results suggest that the IC acts as an operational node containing distinct projection neurons that interpret fear or extinction memories under top-down executive control from the OFC.
To map monosynaptic inputs to IC-CeA and IC-NAc projections, the researchers used rabies virus (RV)-mediated retrograde tracing. They injected retrograde AAV expressing Cre (Retro-AAV-Syn-Cre) into the CeA or NAc of wild-type mice, and injected AAV with Cre-inducible expression of the avian-specific retroviral receptor (TVA) and rabies glycoprotein (RG) into the IC. Four weeks after injection of these AAVs, RV expressing DsRed (RV-ENVA-dG-DsRed) was injected into the IC (Figure 1a). Trans-synaptically-labeled presynaptic neurons (expressing DsRed only) of IC-CeA and IC-NAc projections were found in multiple cortical and subcortical areas, most of which were located in the isocortex (Figure 1b). Within cortical regions, IC-NAc projectors received more top-down input from the frontal cortex (orbitofrontal cortex, OFC, and prelimbic region, PL) than IC-CeA projectors, with input from OFC being approximately three times greater than input from PL. In contrast, IC-CeA projectors received more input from the somatosensory cortex (SS).
Figure 1. Whole-brain mapping of monosynaptic inputs to IC projectors. (Wang Q, et al., 2022)
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
We’re thrilled with the results from Creative Biogene’s AAV vector. Its efficiency in transducing neurons has opened new avenues in our neural circuit studies.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
