Cat.No. : AAV00046Z
Titer: ≥1x10^12 GC/mL / ≥1x10^13 GC/mL Size: 30 ul/100 ul/500 ul/1 ml
Serotype: AAV Serotype 6 Storage: -80 ℃
| Cat. No. | AAV00046Z |
| Description | Cre Adeno-associated virus(AAV Serotype 6) which express Cre recombinase under the control CMV promoter. This product used in the Cre-lox system as a genetic tool to generate site-specific recombination of DNA between loxP sites in cultured cells and animal experiments. |
| Serotype | AAV Serotype 6 |
| Target Gene | cre |
| Titer | Varies lot by lot, typically ≥1x10^12 GC/mL |
| Size | Varies lot by lot, for example, 30 μL, 50 μL, 100 μL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance. |
| Purity | AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE. |
| Sterility | The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities. |
| Empty vs. Full Capsids | Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods. |
In this study, researchers uncovered potential roles for the corticospinal, cerebellar-rubrospinal, and hypothalamic A11 dopaminergic systems in the development of restless legs syndrome (RLS)-like movements during sleep through targeted ablations. Targeted lesions in selective basal ganglia (BG) structures also revealed important roles for nigrostriatal dopamine, striatum, and external globus pallidus (GPe) in modulating RLS-like movements, particularly the pallial cortical projections from the GPe to the motor cortex. Further studies showed that pramipexiole, a dopamine agonist used to treat human RLS, reduced RLS-like movements. Taken together, the data here suggest that BG-cortico-spinal, cerebellar-rubrospinal, and A11 descending projections all contribute to the suppression of motor activity during sleep and sleep-wake transitions, and that disruption of these circuit nodes produces RLS-like movements. These findings provide further support for the concept that the anatomic and genetic etiological bases of RLS are diverse.
Here, to avoid the weight loss caused by bilateral GPe lesions, the researchers performed unilateral GPe lesions in five rats with ibotenic acid (Figure 1). Two weeks later, they recorded EEG/EMG/video for 24 hours.
Figure 1. Histology of GPe and pallial cortical neuronal lesions. GPe lesions were made with ibotenic acid (A). Selective lesions of pallial cortical neurons were produced by injection of AAV6-cre in M2 and the cre-dependent AAV10-DTA in GPe (B-D). Since AAV6-cre was injected only into M2 (C), cell loss in the GPe was limited to pallial cortical neurons projecting to M2 (D), while pallial cortical neurons projecting to other cortical areas were not affected. Arrows point to cre-labeled neurons that were not exposed to AAV-DTA-mCherry, while no cre was seen in the presence of AAV-DTA-mCherry (brown), indirectly indicating that cre-labeled pallial cortical neurons were killed. (Guo C N, et al., 2017)
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