Cat.No. : CSC-RI0207 Host Cell: HEK293
| Cat. No. | CSC-RI0207 |
| Description | This cell line is engineered to stably express mouse transient receptor potential cation channel, subfamily V, member 1 (TRPV1) in HEK293 cells. |
| Gene | TRPV1 |
| Gene Species | Mus musculus (Mouse) |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The TRPV1 ion channel plays a critical role in detecting both thermal and chemical pain sensations. Researchers demonstrate TRPV1's role in thermal and chemical pain sensation. TRPV1 activation leads to Ca2+-dependent desensitization, including acute desensitization and tachyphylaxis. They reveal that the strength of stimulation regulates whole-cell TRPV1 tachyphylaxis. Using light-sheet microscopy and pH-sensitive sensor pHluorin, they observe TRPV1 endocytosis and exocytosis trafficking, linking tachyphylaxis to TRPV1 recycling to the plasma membrane. This activity-dependent trafficking, associated with distinct Ca2+ signals, involves synaptotagmin 1 and 7-mediated recycling routes, influen cing TRPV1 surface expression and nociceptive response.
Figure 1. TRPV1's role in synaptotagmin-mediated regulation of recycling was investigated by researchers. Syt1 facilitated TRPV1 recovery during capsaicin-induced tachyphylaxis. Low capsaicin-evoked TRPV1 recycling was facilitated by Syt1, not Syt7, in HEK cells expressing TRPV1 and Syt1. (Tian Q, et al., 2019)
Using Creative Biogene's Mouse TRPV1 Stable Cell Line - HEK293 in similar experiments could offer several improvements. Firstly, employing a stable cell line ensures consistent expression levels of TRPV1 across experiments, reducing variability. This stability allows for more reliable and reproducible results compared to transient transfections. Furthermore, the use of a stable cell line saves time and resources by eliminating the need for repeated transfections and selection processes, thereby streamlining experimental workflows.
A: HEK293 cells were likely selected for their robust expression machinery and suitability for heterologous expression of ion channels like TRPV1, enabling studies on its functional properties and pharmacology.
A: Stability was likely confirmed through methods such as calcium imaging, patch-clamp electrophysiology, or functional assays assessing TRPV1 activation by capsaicin or other ligands, with continuous selection pressure applied.
A: Characterization may involve analysis of TRPV1 subcellular localization, sensitivity to temperature and ligands, ion channel properties, and functional implications in pain perception and sensory signaling.
A: Quality control likely included screening for mycoplasma contamination, confirmation of stable transgene integration, and assessment of phenotypic stability and consistency.
A: Comparative analysis with sensory neurons or animal models helps validate the relevance of TRPV1 expression in pain pathways and its potential as a target for analgesic drugs or thermoregulatory agents.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Exceptional quality! The Mouse TRPV1 Stable Cell Line in HEK293 cells has been a cornerstone in my pain research. Its stable expression of TRPV1 has provided reliable and reproducible results, facilitating detailed investigations into pain signaling pathways.
Reliable and consistent! The stable expression of TRPV1 in HEK293 cells has ensured the accuracy and precision of my experiments, allowing me to confidently explore the role of TRPV1 in pain perception and nociception.
Streamlining my studies! With the Mouse TRPV1 Stable Cell Line, I've been able to conduct high-throughput screening assays with ease. Its robust TRPV1 expression has simplified data interpretation and accelerated the pace of my research.
Impressive performance! The Mouse TRPV1 Stable Cell Line has exceeded my expectations in terms of reliability and efficiency. Its stable expression of TRPV1 has been invaluable in elucidating the molecular mechanisms underlying thermal and chemical pain sensations.
A valuable research tool! The Mouse TRPV1 Stable Cell Line has significantly advanced my understanding of TRPV1-mediated pain signaling. Its consistent performance has been instrumental in unraveling the complexities of pain perception and sensitization.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
