Cat.No. : CSC-RT0096
Host Cell: caco-2 Target Gene: TRPV1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0096 |
| Description | caco-2-TRPV1 cell line is a stable cell line with a homozygous knockout of TRPV1 |
| Target Gene | TRPV1 |
| Host Cell | caco-2 |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
TRPV1 Knockout Cell Line-caco-2 has truly exceeded my expectations. Its remarkable efficacy and user-friendly design have made gene editing experiments a seamless and successful process.
I am thoroughly impressed with the knockout efficiency of TRPV1 Knockout Cell Line-caco-2. It has consistently delivered precise and reliable results, allowing me to study gene function with confidence.
TRPV1 Knockout Cell Line-caco-2 has become an invaluable asset in my research toolkit. Its efficiency and ease of use have significantly accelerated my experiments and propelled my scientific discoveries.
I cannot emphasize enough how valuable TRPV1 Knockout Cell Line-caco-2 has been in my research. It has streamlined the gene editing workflow, enabling me to achieve knockout results with exceptional precision.
I wholeheartedly recommend TRPV1 Knockout Cell Line-caco-2 to any researcher seeking a reliable and effective tool for gene editing. Its outstanding performance and consistent knockout outcomes have greatly enhanced the quality of my studies.
I am extremely impressed with the knockout efficiency of TRPV1 Knockout Cell Line-caco-2. It has consistently delivered precise and reliable results, empowering me to decipher the roles of specific genes in biological processes.
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