Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT0096
Target Gene : TRPV1 Host Cell : Caco-2
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT0096 |
| Description | caco-2-TRPV1 cell line is a stable cell line with a homozygous knockout of TRPV1 |
| Target Gene | TRPV1 |
| Host Cell | Caco-2 |
| Host Cell Species | Homo sapiens (Human) |
| Size | >1x106 cells/vial |
| Validation | Sequencing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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TRPV1 Knockout Cell Line-caco-2 has truly exceeded my expectations. Its remarkable efficacy and user-friendly design have made gene editing experiments a seamless and successful process.
I am thoroughly impressed with the knockout efficiency of TRPV1 Knockout Cell Line-caco-2. It has consistently delivered precise and reliable results, allowing me to study gene function with confidence.
TRPV1 Knockout Cell Line-caco-2 has become an invaluable asset in my research toolkit. Its efficiency and ease of use have significantly accelerated my experiments and propelled my scientific discoveries.
I cannot emphasize enough how valuable TRPV1 Knockout Cell Line-caco-2 has been in my research. It has streamlined the gene editing workflow, enabling me to achieve knockout results with exceptional precision.
I wholeheartedly recommend TRPV1 Knockout Cell Line-caco-2 to any researcher seeking a reliable and effective tool for gene editing. Its outstanding performance and consistent knockout outcomes have greatly enhanced the quality of my studies.
I am extremely impressed with the knockout efficiency of TRPV1 Knockout Cell Line-caco-2. It has consistently delivered precise and reliable results, empowering me to decipher the roles of specific genes in biological processes.
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