Human TIGIT Stable Cell Line - Jurkat

Name: Human TIGIT Stable Cell Line - Jurkat
SKU: CSC-RO0586
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0586

Host Cell : Jurkat Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0586
Description	This cell line is derived from Jurkat and is engineered to stably overexpress Human TIGIT.
Target Gene	TIGIT
Gene Species	Homo sapiens (Human)
Host Cell	Jurkat
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TIGIT T cell immunoreceptor with Ig and ITIM domains [ Homo sapiens ]
Gene Symbol	TIGIT
Synonyms	TIGIT; T cell immunoreceptor with Ig and ITIM domains; V set and immunoglobulin domain containing 9 , V set and transmembrane domain containing 3 , VSIG9, VSTM3; T-cell immunoreceptor with Ig and ITIM domains; DKFZp667A205; FLJ39873; V-set and transmembrane domain containing 3; V-set and immunoglobulin domain containing 9; Washington University cell adhesion molecule; V-set and transmembrane domain-containing protein 3; V-set and immunoglobulin domain-containing protein 9; VSIG9; VSTM3; WUCAM;
GeneID	201633
Uni ProtID	Q495A1
mRNA Refseq	BC101289
Chromosome Location	3q13.31
Function	protein binding;
MIM	612859

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Background

Case Study

Applications

Q & A

Customer Reviews

The TIGIT gene encodes a protein known as T-cell immunoglobulin and ITIM structural domain protein, which is a member of the immunoglobulin superfamily and is expressed predominantly on T-cells and natural killer cells.TIGIT is involved in the regulation of immune cell activation, proliferation, and cytotoxicity by interacting with receptors such as CD155 and CD159. TIGIT is a member of the T-cell and natural killer cell family. TIGIT has been found to play an important role in a variety of inflammatory and autoimmune diseases.

The Jurkat cell line is a human T-cell leukaemia cell line derived from mature T-cells of a patient.Jurkat cells are easy to culture in vitro and have high proliferative capacity and good growth characteristics. In addition, Jurkat cells express a variety of T-cell receptors and cytokines, making them ideal model cells for studying T-cell biology and signal transduction processes.

Overall, the TIGIT gene and its encoded proteins play a key role in immune regulation. And the Jurkat cell line, as a powerful tool for studying T cells, provides an important platform for revealing the function of TIGIT in immune response. Further study of TIGIT and Jurkat cells may provide new therapeutic strategies for the treatment of inflammatory and autoimmune diseases.

Emerging immunological checkpoint molecule TIGIT (T cell immunoreceptor with Ig and ITIM domains) interacts with ligand PVR (poliovirus receptor) to promote immune tolerance and tumor escape, causing T and NK cell anergy. The scientists used in silico mutagenesis and MD simulations to study TIGIT and PVR. Their findings revealed that specific loops within PVR experienced significant intra-molecular rearrangements during the simulations, resulting in increased hydrogen bonding with TIGIT. They identified key residues for interaction and predicted several high-affinity PVR mutants (PVRS72W, PVRS72R, PVRG131V, and PVRS132Q) through biological assays on TIGIT-overexpressing Jurkat cells. These mutants demonstrated a stronger binding affinity and more potent inhibitory effects compared to the wild-type PVR.

Figure 1 illustrates the IL-2 production in Jurkat-hTIGIT cells co-cultured with CHO-K1, CHO-K1-hPVR, and CHO-K1 mutants, highlighting the frequency of IL-2-secreting cells detected via flow cytometry after 48 hours of stimulation. (doi: 10.1186/s12964-020-00701-y) Figure 1. The researchers investigated the biological functions of PVR mutants by transfecting human TIGIT into Jurkat cells, which do not express endogenous TIGIT. They co-cultured these Jurkat-TIGIT cells with CHO-K1 cells expressing either wild-type or mutant PVR. (Zhou X, et al., 2021)

Creative Biogene's Human TIGIT Stable Cell Line - Jurkat offers a robust platform for further research in this field, allowing researchers to explore the dynamics of TIGIT and its ligands. With stable expression, this cell line enables detailed investigations into the efficacy of novel PVR mutants and their potential therapeutic applications.

Human TIGIT Stable Jurkat Cell Line has shown great potential in scientific research, clinical trials, and translational applications. In research settings, this cell line serves as an invaluable tool for studying the functions and mechanisms of TIGIT, a protein involved in the regulation of immune responses. It allows researchers to investigate the role of TIGIT in various diseases, such as cancer and infections, and to explore potential therapeutic strategies targeting this protein.

In clinical trials, the Human TIGIT Stable Jurkat Cell Line is used to test the efficacy and safety of new immunotherapies. By using this cell line, researchers can evaluate the impact of TIGIT inhibitors on immune cell function and explore their potential to enhance the immune response against tumors. This information is crucial for the development of innovative cancer treatments.

Translationally, the Human TIGIT Stable Jurkat Cell Line enables the translation of basic research findings into clinical practice. It helps in the identification of promising drug candidates and in the assessment of their therapeutic potential. Additionally, this cell line can be used to study the effects of TIGIT inhibitors on immune cell populations in vitro, providing valuable insights into their potential applications in immunomodulatory therapies.

Overall, the Human TIGIT Stable Jurkat Cell Line plays a significant role in advancing our understanding of TIGIT function and its potential as a therapeutic target.

Customer Q&As

What is the TIGIT gene and its function?

A: The TIGIT gene encodes for the TIGIT (T cell immunoglobulin and ITIM domain) protein, which is a recently discovered inhibitory receptor expressed on the surface of T cells and natural killer (NK) cells. TIGIT plays a role in regulating immune responses by inhibiting the activation and function of these immune cells. It does so by interacting with other proteins on the surface of antigen-presenting cells and T cells.

How does the TIGIT gene contribute to immune response regulation?

A: The TIGIT gene contributes to immune response regulation by inhibiting excessive immune activation and preventing autoimmunity. By interacting with the CD226 receptor on T cells and NK cells, TIGIT can inhibit their responses to certain antigens. This inhibition helps maintain immune tolerance and prevents the immune system from attacking healthy cells.

Are there any diseases associated with mutations in the TIGIT gene?

A: Mutations in the TIGIT gene are not commonly associated with specific diseases. However, dysregulation of TIGIT expression or function may lead to alterations in immune responses, potentially affecting the body's ability to mount effective defenses against pathogens or cancer cells.

How is the expression of the TIGIT gene regulated?

A: The expression of the TIGIT gene is regulated by various factors, including cytokines and transcription factors. The activation of T cells and NK cells can lead to the upregulation of TIGIT expression. Additionally, regulatory mechanisms, such as epigenetic modifications and post-translational modifications, can influence the expression of TIGIT, ensuring proper immune responses.

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Customer Reviews

Immunogenicity

The Human TIGIT Stable Cell Line - Jurkat exhibits low immunogenicity, making it suitable for applications where minimal immune response is desired, such as in vitro immunological assays and drug discovery research.

Canada

2021-05-10

Sterility testing

The cell line undergoes rigorous sterility testing to ensure that it is free from any contaminants, providing researchers with a reliable and pure culture for their experiments without the risk of introducing unwanted microbes.

United States

2022-07-14

Tumorigenicity

The Human TIGIT Stable Cell Line - Jurkat is derived from a human T-cell leukemia cell line, and therefore, it is important to handle it with appropriate safety measures. Researchers should be aware of the potential tumorigenic properties and follow ethical guidelines when working with this cell line.

United States

2021-11-12

Mycoplasma testing

Regular mycoplasma testing is performed on the Human TIGIT Stable Cell Line - Jurkat to ensure its integrity and prevent any potential contamination that could affect experimental results. This guarantees the reliability and consistency of the cell line for various research applications.

United Kingdom

2022-09-05

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Human TIGIT Stable Cell Line - Jurkat

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Case Study

Applications

Q & A

Customer Reviews

What is the TIGIT gene and its function?

How does the TIGIT gene contribute to immune response regulation?

Are there any diseases associated with mutations in the TIGIT gene?

How is the expression of the TIGIT gene regulated?

Immunogenicity

Sterility testing

Tumorigenicity

Mycoplasma testing

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