Cat.No. : CSC-RO0709 Host Cell: Jurkat
| Cat. No. | CSC-RO0709 |
| Description | This cell line is engineered to stably express monkey TIGIT. |
| Gene | TIGIT |
| Host Cell | Jurkat |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
MG1131 is the designation of the IgG4-type monoclonal antibody that the researchers created against human TIGIT by employing a phage display library of single-chain variable fragments (scFvs). Compared to PVR, this antibody's ligand, interacts with TIGIT more strongly. It was discovered through crystal structural study that MG1131 successfully prevents PVR and TIGIT from interacting, hence preventing TIGIT's immunosuppressive signaling. Furthermore, MG1131 attaches to cells that express TIGIT, preventing PVR binding. MG1131 restored peripheral blood mononuclear cells' interferon-γ release, suppressed Treg cells' immunosuppressive activity, and improved NK cell-mediated tumor killing in experimental conditions. Moreover, it inhibited the growth of tumors in mice and encouraged T cell infiltration into tumor locations.
Figure 1. The researchers generated a Jurkat cell line that stably expressed TIGIT by cloning the full-length hTIGIT cDNA into the pEF1α-AcGFP-N1 vector and introducing it into Jurkat E6.1 cells. These hTIGIT-Jurkat cell lines were used to screen and identify high-affinity antibodies that can effectively block the binding of PVR to hTIGIT. (Jeong BS, et al., 2022)
A: Our monkey TIGIT stable cell line has undergone rigorous purification and validation procedures to ensure its purity and stability. We use a variety of techniques to identify cell lines, including PCR, immunofluorescence staining, and Western blot. We also regularly perform cytological testing of cell lines to ensure they are free of hybridization or mutation.
A: Our monkey TIGIT stable cell line has stable expression levels and has been widely used in research related to immune regulation. We provide detailed experimental protocols, including recommended experimental steps and technical tips, to help customers use our products in their research.
A: Our monkey TIGIT stable cell line is primarily used for in vitro research, but can also be used in certain in vivo models. We provide supporting data from relevant preclinical studies demonstrating the potential of our products in in vivo models. Customers can refer to this data to understand the performance and range of applications of our products in in vivo studies.
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The quality of Monkey TIGIT Stable Cell Line - Jurkat is very satisfactory. During culture, cells exhibit consistent growth characteristics and stable gene expression, which is critical for studying immune responses, especially T cell biology. The expression of TIGIT gene in Jurkat cells is very stable, which ensures the reliability and consistency of experimental data. Jurkat cells are derived from patients with acute T-cell leukemia. They have typical T-cell characteristics and are very suitable for immunological research. The cell products provided by the supplier are of excellent quality, and the overall experience is very professional, ensuring the smooth progress of the experiment.
From placing the order to receiving the goods, the entire process is efficient and transparent. The customer service staff are very professional, answered my questions in detail, and provided all-round technical support. The cells grew well during the culture process without any contamination or abnormal problems, which made the experiment proceed very smoothly.
Gene expression analysis showed that the expression of TIGIT is very stable, providing reliable data support for studying T cell-mediated immune regulation. Jurkat cells are ideal for complex immunological studies due to their typical T cell properties and ease of manipulation. The supplier's delivery speed is fast and the packaging is professional, ensuring the viability of the cells.
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