Cat.No. : CSC-RO0040 Host Cell: CHO-K1
| Cat. No. | CSC-RO0040 |
| Description | This cell line is a stably transfected cell line which expresses Human TIGIT without any tag. |
| Gene | TIGIT |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | TIGIT T cell immunoreceptor with Ig and ITIM domains [ Homo sapiens ] |
| Gene Symbol | TIGIT |
| Synonyms | TIGIT; T cell immunoreceptor with Ig and ITIM domains; V set and immunoglobulin domain containing 9 , V set and transmembrane domain containing 3 , VSIG9, VSTM3; T-cell immunoreceptor with Ig and ITIM domains; DKFZp667A205; FLJ39873; V-set and transmembrane domain containing 3; V-set and immunoglobulin domain containing 9; Washington University cell adhesion molecule; V-set and transmembrane domain-containing protein 3; V-set and immunoglobulin domain-containing protein 9; VSIG9; VSTM3; WUCAM; |
| Gene ID | 201633 |
| UniProt ID | Q495A1 |
| mRNA Refseq | BC101289 |
| Chromosome Location | 3q13.31 |
| Function | protein binding; |
| MIM | 612859 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Myelodysplastic syndrome (MDS) comprises heterogeneous blood disorders, originating from abnormal hematopoietic stem cells. It features peripheral blood (PB) cytopenia, bone marrow (BM)dysfunctional hematopoiesis, and increased AML risk. Researchers aimed to investigate the role of TIGIT in myelodysplastic syndrome (MDS) and its potential as a therapeutic target. They obtained fresh PB and BM samples from MDS patients and healthy donors, analyzing TIGIT expression on NK and T cells using flow cytometry (FCM) and PCR. Results showed high TIGIT expression on PB NK and T cells, correlating with disease progression and immune evasion in MDS. Functional assays revealed impaired NK and T cell activity associated with elevated TIGIT levels. Notably, blocking TIGIT enhanced antitumor effects, suggesting its potential as a therapeutic target in MDS.
Figure 1. The function of NK and T cells is limited by TIGIT. Cytokine expression and proliferation of TIGIT+ and TIGIT– NK and T cells were assessed in HDs. (Meng F, et al., 2020)
Utilizing Creative Biogene's Human TIGIT Stable CHO Cell Line enhances experiments related to TIGIT function. This stable cell line offers a consistent and reliable cellular model for investigating TIGIT-mediated immune regulation. By using this cell line, researchers can streamline experimental procedures, reduce variability, and achieve reproducible results in studies focused on TIGIT's impact on NK and T cell function, cytokine expression, and proliferation.
A: SH-SY5Y cells were likely chosen for their neuroblastoma origin and relevance to neuroscience research, providing a model system to investigate gene function and regulatory mechanisms using CRISPR/Cas9 technology.
A: Efficiency and specificity were likely assessed through methods such as genomic sequencing, functional assays measuring indel formation, or reporter assays targeting known Cas9 cleavage sites.
A: Characterization may involve analysis of knockout or knock-in efficiency, off-target effects, changes in neuronal differentiation, synaptic function, or response to neurotoxic stimuli upon genetic manipulation.
A: Quality control likely included confirmation of Cas9 expression levels, validation of its editing efficiency and specificity, assessment of off-target effects, and validation of phenotypic changes associated with genetic manipulation.
A: Comparative analysis with other neuronal models or patient-derived samples helps elucidate the functional significance of targeted genes in neurological disorders, identify potential therapeutic targets, and guide drug development efforts.
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Exceptional quality! The Human TIGIT Stable Cell Line in CHO cells has been invaluable in our immunology research. Its stable expression of TIGIT has provided a reliable model for investigating T cell inhibition and immune checkpoint regulation.
Reliable and consistent! The stable expression of TIGIT in CHO cells has facilitated precise and accurate studies of immune checkpoint pathways. Its consistent TIGIT expression has ensured reproducible results and enhanced the reliability of our experiments.
Streamlining our studies! With the Human TIGIT Stable Cell Line, we've been able to explore the therapeutic potential of targeting immune checkpoints with confidence. Its stable expression of TIGIT has simplified experimental workflows and accelerated the pace of our research.
Impressive performance! The Human TIGIT Stable Cell Line has consistently exhibited robust TIGIT expression, exceeding our expectations. Its stable expression has been instrumental in elucidating the role of TIGIT in immune evasion and antitumor immunity.
A valuable tool in immunotherapy! The Human TIGIT Stable Cell Line has transformed our understanding of immune regulation. Its stable expression of TIGIT has provided a solid foundation for investigating novel immunotherapeutic strategies and advancing the development of immune checkpoint inhibitors.
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